Fixation of histology samples.- Processing of tissue.- Embedding of tissue.- Decalcification of bone.- Tissue microtomy.- Frozen section of tissue: technique and applications.- Staining principle and general procedure of stain.- Haematoxylin and eosin stain.- Special stains for protein, lipid, carbohydrate, nucleic acid and pigments.- Stains for the connective tissue.- Stains for the amyloid.- Stains for the microbial organisms.- Cytology sample: procurement, fixation and processing.- Routine staining in cytology laboratory.- Fine needle aspiration biopsy technique.- Immunochemistry in histology and cytology: principle, technique and applications.- Flow cytometry: principle, technique and applications.- Digital image analysis and virtual microscopy: principle, technique and applications.- Liquid based cytology and automated screening devices.- Polymerase chain reaction and DNA sequencing: principle, technique and applications.- Fluorescent in situ hybridisation techniques: principle, technique and applications.- Tissue microarray: technique and applications.- Sanger sequencing and next generation gene sequencing: basic principles and applications.- Light microscope: optical components, principle and handling.- Fluorescence microscope and confocal microscopy: basic parts and principle.- Laboratory organization and quality control.- Laboratory safety: essential precautions.
This book provides detailed information on basic and advanced laboratory techniques in histopathology and cytology. It discusses the principles of and offers clear guidance on all routine and special laboratory techniques. In addition, it covers various advanced laboratory techniques, such as immunocytochemistry, flow cytometry, liquid based cytology, polymerase chain reaction, tissue microarray, and molecular technology. Further, the book includes numerous color illustrations, tables and boxes to familiarize the reader with the work of a pathology laboratory.
The book is mainly intended for postgraduate students and fellows in pathology as well as practicing pathologists. The book is also relevant for all the laboratory technicians and students of laboratory technology.
Dr. Pranab Dey is a professor at the department of cytology and gynecologic pathology at the Postgraduate Institute of Medical Education and Research, Chandigarh. Professor Dey completed his MBBS at R. G. Kar Medical College and Hospital, Calcutta, M.D. (Pathology) at the Postgraduate Institute of Medical Education and Research, Chandigarh and his FRCPath (Cytopathology) at the Royal College of Pathologists, London. Professor Dey has conducted several research projects and has pioneered work on DNA flow cytometry, image morphometry, mono-layered cytology and cytomorphologic findings in various lesions in cytology smears. He has written numerous publications and is a member of various societies.