Molecular Cloning. A Laboratory Manual, 3 Vols.

Description

Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.Molecular Cloning, Fourth Edition, by the celebrated founding author Joe Sambrook and new co-author, the distinguished HHMI investigator Michael Green, preserves the highly praised detail and clarity of previous editions and includes specific chapters and protocols commissioned for the book from expert practitioners at Yale, U Mass, Rockefeller University, Texas Tech, Cold Spring Harbor Laboratory, Washington University, and other leading institutions. The theoretical and historical underpinnings of techniques are prominent features of the presentation throughout, information that does much to help trouble-shoot experimental problems.

For the fourth edition of this classic work, the content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories.

Core chapters from the third edition have been revised to feature current strategies and approaches to the preparation and cloning of nucleic acids, gene transfer, and expression analysis. They are augmented by 12 new chapters which show how DNA, RNA, and proteins should be prepared, evaluated, and manipulated, and how data generation and analysis can be handled.

The new content includes methods for studying interactions between cellular components, such as microarrays, next-generation sequencing technologies, RNA interference, and epigenetic analysis using DNA methylation techniques and chromatin immunoprecipitation. To make sense of the wealth of data produced by these techniques, a bioinformatics chapter describes the use of analytical tools for comparing sequences of genes and proteins and identifying common expression patterns among sets of genes.

Building on thirty years of trust, reliability, and authority, the fourth edition ofMolecular Cloning is the new gold standard—the one indispensable molecular biology laboratory manual and reference source.

Highlights of the new edition:

• Extensive new content: 12 entirely new chapters are devoted to the most exciting current research strategies, including epigenetic analysis, RNA interference, genome sequencing, and bioinformatics
• Expanded scope: the nucleic-acid-based techniques selected for inclusion have promoted recent advances in gene transfer, protein expression, RNA analysis, and expression of cloned genes
• Classic content: 10 original core chapters have been updated to reflect developments and innovations in standard techniques and to introduce new cutting-edge protocols
• Easy-to-follow format: the previous editions’ renowned attention to detail and accuracy are fully retained
• Essential appendices: an up-to-date collection of reagents, vectors, media, detection systems, and commonly used techniques are included
• Expanded authorship: chapters and protocols have been specifically commissioned from renowned experts at leading institutions

Reviews

“Any basic research laboratory using molecular biology techniques will benefit from having a copy on hand of the newly published Third Edition of Molecular Cloning: A Laboratory Manual...the first two editions of this book have been staples of molecular biology with a proven reputation for accuracy and thoroughness.”
—The Scientist

“In every kitchen there is at least one indispensable cookbook...Molecular Cloning: A Laboratory Manual fills the same niche in the laboratory (with) information to help both the inexperienced and the advanced user. (It) has once again established its primacy as the molecular laboratory manual and is likely to be found on lab benches...around the world.”
——Trends in Neurosciences

“Molecular Cloning: A Laboratory Manual has always been the laboratory mainstay for protocols and techniques. It has a pure-bred ancestry, and the new edition does not disappoint. (It) includes information panels at the end of each chapter that describe the principles behind the protocols.... The addition of this information extends Molecular Cloning from an essential laboratory resource into a new realm, one merging the previous prototype with a modern molecular monograph...the next generation of Molecular Cloning not only carries on the proud heritage of the first two editions but also admirably expands on that tradition to provide a truly essential laboratory manual.”
—Trends in Microbiology

Contents

VOLUME 1

• Part 1: Essentials
• 1. Isolation and Quantification of DNA
• 2. Analysis of DNA
• 3. Cloning and Transformation with Plasmid Vectors
• 4. Gateway Recombinational Cloning
  John S. Reece-Hoyes and Albertha J.M. Walhout
• 5. Working with Bacterial Artificial Chromosomes and Other High-Capacity Vectors
  Nathaniel Heintz and Shiaoching Gong
• 6. Extraction, Purification, and Analysis of RNA from Eukaryotic Cells
• 7. Polymerase Chain Reaction
• 8. Bioinformatics
  Jui-Hung Hung and Zhiping Weng

VOLUME 2

• Part 2: Analysis and Manipulation of DNA and RNA
• 9. Quantification of DNA and RNA by Real-Time Polymerase Chain Reaction
• 10. Nucleic Acid Platform Technologies
  Oliver Rando
• 11. DNA Sequencing
  Elaine Mardis and W. Richard McCombie
• 12. Analysis of DNA Methylation in Mammalian Cells
  Paul M. Lizardi, Qin Yan, and Narendra Wajapeyee
• 13. Preparation of Labeled DNA, RNA, and Oligonucleotide Probes
• 14. Methods for In Vitro Mutagenesis
  Matteo Forloni, Alex Liu, and Narendra Wajapeyee
• Part 3: Introducing Genes into Cells
• 15. Introducing Genes into Cultured Mammalian Cells
  Priti Kumar, Arvindhan Nagarajan, and Pradeep D. Uchil
• 16. Introducing Genes into Mammalian Cells: Viral Vectors
  Guangping Gao and Miguel Sena-Esteves

VOLUME 3

• Part 4: Gene Expression
• 17. Analysis of Gene Regulation Using Reporter Systems
• Pradeep D. Uchil, Arvindhan Nagarajan, and Priti Kumar
• 18. RNA Interference and Small RNA Analysis
• Chengjian Li and Phillip D. Zamore
• 19. Expressing Cloned Genes for Protein Production, Purification, and Analysis
• Clara L. Kielkopf, William Bauer, and Ina Urbatsch
• Part 5: Interaction Analysis
• 20. Cross-Linking Technologies for Analysis of Chromatin Structure and Function
• Tae Hoon Kim and Job Dekker
• 21. Mapping of In Vivo RNA-Binding Sites by UV-Cross-Linking Immunoprecipitation (CLIP)
• Jennifer C. Darnell, Aldo Mele, Ka Ying Sharon Hung, and Robert B. Darnell
• 22. Gateway-Compatible Yeast One-Hybrid and Two-Hybrid Assays
• John S. Reece-Hoyes and Albertha J.M. Walhout
• Appendices
• 1. Reagents and Buffers
• 2. Commonly Used Techniques
• 3. Detection Systems
• 4. General Safety and Hazardous Material

Author

By Michael R. Green, Howard Hughes Medical Institute, University of Massachusetts Medical School; Joseph Sambrook, Peter MacCallum Cancer Institute, Melbourne, Australia