Manipulating the Mouse Embryo. a Laboratory Manual (Hardback)

Description

This 4th edition of the “Mouse Manual”—Manipulating the Mouse Embryo—appears 28 years after the first edition and once again is the definitive reference source on mouse development, transgenesis techniques, and molecular biology. Authors Richard Behringer, Marina Gertsenstein, Kristina Nagy, and Andras Nagy—pre-emininent leaders in their fields—have reorganized and updated this edition to include new information and protocols on:

• assisted reproduction techniques for sperm and embryo cryopreservation
• generation of induced pluripotent stem cells
• isolation, generation, and transplantation of spermatogonial stem cell lines
• in utero electroporation of gene constructs into post-implantation embryos
• vibratome sectioning of live and fixed tissues for imaging thick tissue sections
• whole-mount fluorescent staining methods for three-dimensional visualization.

Techniques regarding recombinant DNA technology and mouse embryonic development from the previous editions have been updated and recast, as has the wealth of information on mouse laboratory strains, mouse housing and breeding, surgical procedures, assisted reproduction, handling of embryos, and micromanipulation setups. The first edition of Manipulating the Mouse Embryoappeared in 1986 as an outgrowth of Cold Spring Harbor Laboratory courses on the molecular embryology of the mouse held in the early 1980s, and authors of the first two editions included Brigid Hogan, Rosa Beddington, Frank Costantini, and Liz Lacy. Mouse embryo manipulation techniques have developed exponentially since the first edition, but then, as now, Manipulating the Mouse Embryo remains the essential practical and theoretical guide for anyone working with mice—students, lab technicians, and investigators.

Contents

Chapter 1 Genetics and Embryology of the Mouse: Past, Present, and Future
Chapter 2 Summary of Mouse Development
Chapter 3 A Mouse Colony for the Production of Transgenic and Chimeric Animals
Chapter 4 Recovery and In Vitro Culture of Preimplantation Embryos
Chapter 5 Isolation, Culture, and Manipulation of Postimplantation Embryos
Chapter 6 Surgical Procedures
Chapter 7 Production of Transgenic Mice by Pronuclear Microinjection
Chapter 8 Embryo-derived Stem Cell Lines
Chapter 9 Germ Line–Competent Stem Cells Derived from Adult Mice
Chapter 10 Vector Designs for Pluripotent Stem Cell-based Transgenesis and Genome Alterations
Chapter 11 Introduction of Foreign DNA into Embryonic Stem Cells
Chapter 12 Production of Chimeras
Chapter 13 Genotyping
Chapter 14 Parthenogenesis, Pronuclear Transfer, and Mouse Cloning
Chapter 15 Assisted Reproduction: Ovary Transplantation, In Vitro Fertilization, Artificial Insemination, and Intracytoplasmic Sperm Injection
Chapter 16 Cryopreservation, Rederivation, and Transport of Mouse Strains
Chapter 17 Techniques for Visualizing Gene Products, Cells, Tissues, and Organ Systems
Chapter 18 Setting Up a Micromanipulation Lab

Appendices:
Buffers & Solutions
Web Resources
Cautions

By Richard Behringer, University of Texas, M.D. Anderson Cancer Centre; Marina Gertsenstein, Toronto Centre for Phenogenomics, Transgenic Core and Specialty Resources; Kristina Nagy, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto; Andras Nagy, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto