Whole Genome Amplification

Recent advances in the study and understanding of human disease have largely been made possible by advances in molecular biology methodology. However, as the number and variety of laboratory techniques increases, so does the requirement for sufficient quantities of genomic DNA. This shortfall in availability of DNA has been addressed by the development of a number of whole genome amplification (WGA) approaches. Using these methods, it is possible to generate microgram quantities of DNA starting with as little as one nanogram of genomic DNA and in some cases even a single eukaryotic or bacterial cell. The implementation of such WGA methods provides an ample supply of DNA for large-scale genetic studies. This book will be welcomed by experienced researchers wishing to take advantage of the latest developments and by newcomers to the field who need to rapidly identify reliable techniques and approaches that will yield success in the laboratory.

Contents:

• 1. Introduction to whole genome amplification
  José M. Lage and Paul M. Lizardi, both at Yale University School of Medicine, USA
• 2. Single nucleotide polymorphism typing using degenerate-oligonucleotide-primed PCR-amplified products
  Makoto Bannai, Olympus Corporations, Tokyo, Japan and Katsushi Tokunaga, University of Tokyo, Japan
• 3. Whole genome amplification by improved primer-extension pre-amplification PCR
  Peter J. Wild and Wolfgang Dietmaier, both at University of Regensburg, Germany
• 4. Global amplification using SCOMP: single-cell comparative genomic hybridization
  Nona C.R. Arneson, Ontario Cancer Institute, Canada, Arezou A. Ghazani, Unversity of Toronto, Canada and Susan J. Done, Ontario Cancer Institute, Canada
• 5. PRSG, a whole genome amplification method based on adaptor-ligation PCR of randomly sheared genomic DNA
  Hiroki Sasaki and Kazuhiko Aoyagi, both at National Cancer Research Institute, Japan
• 6. GenomePlex whole genome amplification
  Simon Hughes, Gabrielle Sellick, Richard Coleman, all Institute of Cancer Research, UK and John Langmore, Rubicon Genomics, Ann Arbor, USA
• 7. DNA linear amplification Chih Long Liu, Bradley E. Bernstein and Stuart L. Schreiber, all at Harvard University, USA
• 8. Multiple displacement amplification of genomic DNA
  Roger Lasken, Allegheny-Singer Research Institute, Pittsburgh, USA
• 9. Multiple displacement amplification from single bacterial cells
  Roger S. Lasken, Allegheny-Singer Research Institute, Pittsburgh, USA; Arumugham Raghunathan, Qiagen Inc, Valencia, USA; Thomas Kvist, Biocentrum-DTU, Denmark; Thomas Ishøy, Allegheny-Singer Research Institute, Pittsburgh, USA; Peter Westermann, Biocentrum-DTU, Denmark; Birgitte K. Ahring, Biocentrum-DTU, Denmark and Robert Boissy, Allegheny-Singer Research Institute, Pittsburgh, USA
• 10. Genome amplification tolerant to sample degradation: application to formalin-fixed, paraffin-embedded specimens
  G. Mike Makrigiorgos, Dana Farber/Brigham and Women’s Cancer Center, Boston, USA
• 11. Pre-implantation genetic diagnosis using whole genome amplification
  Alan H. Handyside, London Bridge Fertility, UK, Mark D. Robinson, University of Leeds, UK and Francesco Fiorentino, GENOMA, Rome,
  Italy
• Index