Cell Imaging

Cell imaging techniques and applications are now very widely used in modern biology and have become key tools for bioscience researchers. This book focuses on the techniques that people would wish to use on a regular basis in labs that may not currently specialise in imaging but have the capacity to use this technology as a regular component of their work.

Contents:

• 1. An introduction to cell imaging
  David Stephens, University of Bristol, UK
• 2. Confocal or wide-field? A guide to selecting appropriate
  methods for cell imaging
  Mark A. Jepson, University of Bristol, UK
• 3. Image quantification and analysis
  Kota Miura and Jens Rietdorf, both at European Molecular Biology Laboratory, Heidelberg, Germany
• 4. Transmitted light imaging
  Jon D. Lane, University of Bristol, UK
  and Howard Stebbing, Peninsula Medical School, Exeter, UK
• 5. Spectral imaging techniques for fluorescence microscopy Timo Zimmermann, European Molecular Biology Laboratory, Heidelberg, Germany
• 6. Measurement of protein motion by photobleaching
  John F. Presley, MCGill University, Quebec, Canada
• 7. Imaging calcium and calcium-binding proteins
  Burcu Hasdemir, University of California, USA,
  Robert Burgoyne, University of Liverpool, UK
  and Alexei Tepikin, University of Liverpool, UK
• 8. Total internal reflection fluorescence microscopy David Zenisek, Yale University, USA
• 9. An overview of three-dimensional and four-dimensional
  microscopy by computational deconvolution
  José-Angel Conchello, Oklahoma Medical Research Foundation, USA
• 10. Correlative microscopy using Tokuyasu cryosections: applications for immunolabeling and in situ hybridization
  Miguel R. Branco, Sheila Q. Xie, Sonya Martin and Ana Pombo, all at Imperial College, London, UK
• 11. Fluorescence lifetime imaging
  Klaus Suhling, King’s College London, UK
• 12. Homo-FRET measurements to investigate molecular-scale
  organization of proteins in living cells
  Rajat Varma, New York University School of Medicine, USA and Satyajit Mayor, National Centre for Biological Science, Bangalore, India
• 13. High-content and high-throughput screening Elizabeth P. Roquemore, GE Healthcare Biosciences, Cardiff, UK
• List of suppliers
• Index