Protein Engineering. Design, Selection & Applications (Protein Science & Engineering Series)

Description

Protein engineering is the process of developing useful or valuable proteins. It is a young discipline, with much research currently taking place into the understanding of protein folding and protein recognition for protein design principles. There are two general strategies for protein engineering. The first is known as rational design, in which the scientist uses detailed knowledge of the structure and function of the protein to make desired changes. The second strategy is known as directed evolution and this is where random mutagenesis is applied to a protein, and a selection regime is used to pick out variants that have the desired qualities. This book presents and reviews important data on protein engineering, such as application of engineered proteins and cell adhesive surfaces as scaffolds or other biomedical devices which has the potential to promote tissue repair and regeneration for a wide variety of tissues including bone and skin.

Table of Contents

Preface; Synthesizing Mammalian Cell Adhesive Surfaces using Self-Assembling Fibronectin Domains; Engineering Thermobifida fusca Cellulases: Catalytic Mechanisms & Improved Activity; An Empirical Study for Finding a Robust Ensemble of Classifiers for Protein Classification; Revolutionary Protein Engineering Using Molecular Display; Chemoselective Modification using Aldehyde/ketone Tags; Computer Modeling of the Promising HIV-1 Entry Inhibitors for Structure-Based Anti-AIDs Drug Design; Molecular Mechanisms of Protein Folding by Mammalian ER Chaperones & Stress Response; The Use of Protein Engineering Methods for Studies of Calcium Binding Proteins; Index.