Liver Proteomics. Methods and Protocols (Methods in Molecular Biology, Vol. 909)

Description

• Outlines strategies used to characterize liver proteome at the global, cellular, subcellular, post translational, and functional level
• Provides step-by-step detail essential for reproducible results
• Contains key notes and implementation advice from the experts

The liver is responsible for a wide range of critical functions essential to life, and is composed of several different cell types. In Liver Proteomics: Methods and Protocols, expert researchers in the field detail many of the methods that are used to study the live. These methods include the most up-to-date strategies being used to characterize the liver proteome at the global, cellular, subcellular, post translational and functional level.Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Liver Proteomics: Methods and Protocols seeks to aid scientists in the further study of this crucially important organ.

Content Level » Professional/practitioner
Keywords » Golgi complexes - Human Liver Proteome Project - cell types - hepatocyte - liver - metabolomic profiling - post translational - post translational modifications - proteins
Related subjects » Biochemistry & Biophysics - Molecular Medicine

TABLE OF CONTENTS

1. Cell Surface Capturing (CSC) technologies for the surfaceome discovery of hepatocytes
   Damaris Bausch-Fluck, Hofmann Andreas, and Bernd Wollscheid
2. An on-target desalting and concentration sample preparationprotocol for MALDI-MS and MS/MS analysis
   Xumin Zhang , Quanhui Wang, Xiaomin Lou, Haidan Sun, Peter Roepstorff, and Siqi Liu
3. Plasma membrane isolation using immobilized concanavalin A magnetic beads
   Yu-Chen Lee, Martina Srajer Gajdosik, Djuro Josic and Sue-Hwa Lin
4. Analysis of the cattle liver proteome by High-sensitive Liquid Chromatography Coupled with Mass Spectrometry method
   Anna Maria Timperio, Gian Maria D’Amici, Lello Zolla
5. Producing a One-Dimensional Proteomic Map for Human Liver Cytochromes P450
   Natalia A. Petushkova and Andrey V. Lisitsa
6. Assessing heterogeneity of peroxisomes – isolation of two subpopulations from rat liver
   Markus Islinger, Afsaneh Abdolzade-Bavil, Sven Liebler, Gerhardt Weber, Alfred Völkl
7. Purification and Proteomic Analysis of Liver Membrane Skeletons
   Jintang He, Yashu Liu, Qingsong Wang and Jianguo Ji
8. Liver Plasma Membranes: An effective method to analyze membrane proteome
   Rui Cao and Songping Liang
9. Quantitative analysis of liver Golgi proteome in the cell cycle
   Xuequn Chen1, Philip C. Andrews2 and Yanzhuang Wang3
10. Glycoproteins and Glycosylation: Apolipoprotein C3 Glycoforms by top-down MALDI-TOF Mass Spectrometry
    Yan Zhang, Alan R. Sinaiko, Gary L. Nelsestuen
11. Phosphoproteomic Analysis of Liver Homogenates
    Gokhan Demirkan, Arthur R. Salomon, and Philip A. Gruppuso
12. A combination of affinity chromatography, 2D DIGE and mass spectrometry to analyze the phosphoproteome of liver progenitor cells
    Enrique Santamaría, Virginia Sánchez-Quiles, Joaquín Fernández-Irigoyen1 and Fernando J. Corrales
13. Identifying Acetylated Proteins in Mitosis
    Eva Rodriguez-Suarez, Jose M. Mato and Felix Elortza
14. Comparative proteome analysis of a human liver cell line stably transfected with Hepatitis D virus full-length cDNA
    Celso Cunha and Ana V. Coelho
15. Identification of new autoimmune hepatitis-specific autoantigens by using protein microarray technology
    Lin Wu, and Guang Song
16. Proteomics analysis of human non-alcoholic fatty liver
    Eva Rodriguez-Suarez, Jose M. Mato and Felix Elortza
17. Proteomic Methods for Biomarker Discovery in a Rat Model of Alcohol Steatosis
    Billy W. Newton, William K. Russell, David H. Russell, Shashi K. Ramaiah, and Arul Jayaraman
18. Targeted mass spectrometry-based metabolomic profiling through Multiple Reaction Monitoring of Liver and other biological matrices
    Angelo D’Alessandro, Federica Gevi,, Lello Zolla
19. Discovery of Lamin B1 and Vimentin as Circulating Biomarkers for Early Hepatocellular Carcinoma
    Kwong-Fai Won1 and John M. Luk
20. Combinatorial peptide ligand libraries to discover liver disease biomarkers in plasma samples
    Gian Maria D'Amici, Anna Maria Timperio, Sara Rinalducci, Lello Zolla
21. Isolation of urinary exosomes from animal models to unravel non-invasive disease biomarkers”
    Javier Conde-Vancells and Juan M. Falcon-Perez