Nanoparticles in Biology and Medicine. Methods and Protocols (Methods in Molecular Biology, Vol. 906)

Description

• Supplies cutting-edge laboratory protocols involving nanoparticles and their applications
• Includes methods on fabrication and synthesis, manufacturing, and characterization
• Features expert tips and key implementation advice to guarantee successful results

The modern fascination with micro- and nano-sized materials can actually be traced back further to the 1960s and ‘70s when the first few reported attempts were made to use nanoparticles for controlled drug delivery. In Nanoparticles in Biology and Medicine: Methods and Protocols, experts in the field present a wide range of methods for synthesis, surface modification, characterization, and application of nano-sized materials (nanoparticles) in life science and medical fields, mostly for drug delivery. The methods presented cover all stages of nanoparticle manufacturing, modification, analysis, and applications. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Nanoparticles in Biology and Medicine: Methods and Protocols will help the beginner become familiar with this fascinating field and will provide scientists at all levels of expertise with easy-to-follow practical advice needed to make, modify, and analyze nanoparticles of their choice and to use them in a wide range of biomedical and pharmaceutical applications, including functional protein studies, drug delivery, immunochemistry, imaging, and many others.

Content Level » Professional/practitioner
Keywords » Drug delivery - Nano-sized materials - Nanoparticle manufacturing - Nanoparticles - Pharmaceutical applications - Surface modification
Related subjects » Pharmaceutical Science

TABLE OF CONTENTS

Part I: Noble Metal Nanoparticles: Synthesis and Applications

• 1. Procedures for the Synthesis and Capping of Metal Nanoparticles
  Claudia Gutiérrez-Wing, J. Jesús Velázquez-Salazar, and Miguel José-Yacamán
• 2. Preparation of Concentrated Colloids of Gold Core-Silica Shell Nanoparticles for Biomedical Applications
  Yeon-Su Park
• 3. Biosynthesis of Silver and Gold Nanoparticles Using Bacillus licheniformis
  Muthu Irulappan Sriram, Kalimuthu Kalishwaralal, and Sangiliyandi Gurunathan
• 4. Gold Nanoparticle Antibody Conjugates for Use in Competitive Lateral Flow Assays
  Julian Bailes, Samantha Mayoss, Phil Teale, and Mikhail Soloviev
• 5. Gold Nanoparticles for Immunogold Localization of Antigens in Plant Tissues
  Sadaki Yokota
• 6. RNA Quantification Using Noble Metal Nanoprobes: Simultaneous Identification of Several Different mRNA Targets Using Colour Multiplexing and Application to Cancer Diagnostics
  Joao Conde, Goncalo Doria, Jesus M. de la Fuente, and Pedro Viana Baptista
• 7. Effect of Gold Nanoparticle Conjugation on the Activity and Stability of Functional Proteins
  Julian Bailes, Sara Gazi, Rositsa Ivanova, and Mikhail Soloviev

Part II: Semiconductor Nanoparticles: Synthesis and Applications

• 8. Synthesis of Semiconductor Nanoparticles
  Xianfeng Chen and Peter J. Dobson
• 9. Size-Selective Synthesis of Ultrasmall Hydrophilic CdSe Nanoparticles in Aqueous Solution at Room Temperature
  Yeon-Su Park, Yukihiro Okamoto, Noritada Kaji, Manabu Tokeshi, and Yoshinobu Baba
• 10. Synthesis and Functionalization of CdSe/ZnS QDs Using the Successive Ion Layer Adsorption Reaction and Mercaptopropionic Acid Phase Transfer Methods
  Ana Sofia Miguel, Christopher Maycock, and Abel Oliva
• 11. In Vivo, Site-Specific, Covalent Conjugation of Quantum Dots to Proteins Via Split-Intein Splicing
  Anna Charalambous, Maria Andreou, Ioanna Antoniades, Neophytos Christodoulou, and Paris A. Skourides
• 12. Evaluating the Potential of Quantum Dots for In Vitro Biological Studies: Effects on Gene Expression Using Microarray Analysis
  Babu R. Prasad, Enda O’ Connell, Terry J. Smith, Valérie A. Gérard, Yurii K. Gun’ko, and Yury Rochev
• 13. The Use of Quantum Dots for Immunochemistry Applications
  Helena Montón, Mónica Roldán, Arben Merkoçi, Emma Rossinyol, Onofre Castell, and Carme Nogués
• 14. Quantum Dots for Labelling Live Cells
  Yun Chen
• 15. Labeling and Imaging Mesenchymal Stem Cells with Quantum Dots
  Maria C. Collins, Philip R. Gunst, Wayne E. Cascio, Alan P. Kypson, and Barbara J. Muller-Borer
• 16. The Application of Semiconductor Quantum Dots for Enhancing Peptide Desorption, Improving Peak Resolution and Sensitivity of Detection in Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry
  Julian Bailes and Mikhail Soloviev

Part III: Magnetic Nanoparticles: Synthesis and Applications

• 17. Near-Infrared Fluorescence Labeling of Iron Nanoparticles and Applications for Cell Labeling and In Vivo Imaging
  Jinke Wang, Yingxun Liu, Yong Hou, Zhongpin Chen, and Ning Gu
• 18. Labeling Stem Cells with Superparamagnetic Iron Oxide Nanoparticles: Analysis of the Labeling Efficacy by Microscopy and Magnetic Resonance Imaging
  Jasmin, Ana Luiza Machado Torres, Linda Jelicks, Antonio Carlos Campos de Carvalho, David C. Spray, and Rosalia Mendez-Otero
• 19. Non-Invasive Quantification of Magnetic Nanoparticles by Means of Magnetorelaxometry
  Maik Liebl and Dietmar Eberbeck
• 20. Synthesis, Application, and Tracking of Magnetic Carbon-Coated Nanoparticles in Plants
  Alejandro Pérez-de-Luque, Zuny Cifuentes, Clara Marquina, Jesús M. de la Fuente, and M. Ricardo Ibarra

Part IV: Organic, Polymeric, Biomimetic, and Biocompatible Nanoparticles and Their Applications

• 21. Synthesis of Biocompatible Gelatinated Thioglycolic Acid-Capped CdTe Quantum Dots (“Jelly Dots”)
  Valérie A. Gérard, Yurii K. Gun’ko, Babu R. Prasad, and Yury Rochev
• 22. Preparation and Characterization of Biomimetic Nanoparticles for Drug Delivery
  Ana Maria Carmona-Ribeiro
• 23. Preparation of Nanoparticles by Spray-Drying and Their Use for Efficient Pulmonary Drug Delivery
  Hao-Ying Li and Fan Zhang
• 24. Synthesis and Evaluation of Airway Targeted PLGA Nanoparticles for Drug Delivery in Obstructive Lung Diseases
  Neeraj Vij
• 25. Preparation of siRNA-Encapsulated PLGA Nanoparticles for Sustained Release of siRNA and Evaluation of Encapsulatiuon Efficiency
  Panayotis Pantazis, Konstantinos Dimas, James H. Wyche, Shrikant Anant, Courtney W. Houchen, Jayanth Panyam, and Rama P. Ramanujam
• 26. Synthesis of Poly (2-hydroxyethyl methacrylate) (PHEMA) Based Nanoparticles for Biomedical and Pharmaceutical Applications
  Rajesh Saini, Jaya Bajpai, and Anil K. Bajpai
• 27. Preparation of Lipid:Peptide:DNA (LPD) Nanoparticles and Their Use for Gene Transfection
  Fan Zhang and Hao-Ying Li
• 28. Crotamine: A Novel Cell-Penetrating Polypeptide Nanocarrier with Potential Anti-Cancer and Biotechnological Applications
  Mirian A.F. Hayashi, Eduardo B. Oliveira, Irina Kerkis, and Richard L. Karpel
• 29. Elastin-Based Nanoparticles for Delivery of Bone Morphogenetic Proteins
  Raul Machado, Paulo C. Bessa, Rui L. Reis, José Carlos Rodriguez-Cabello, and Margarida Casal
• 30. Synthesis, Surface Modification, Characterization, and Biomedical In Vitro Applications of Organically Modified Silica (ORMOSIL) Nanoparticles
  Diksha and Indrajit Roy
• 31. Extraction of Organic Nanoparticles from Plants
  Scott C. Lenaghan, Qin Zhu, Lijin Xia, and Mingjun Zhang

Part V: Nano-Toxicity and Nano-Safety

• 32. Assessment of Cytotoxicity of Carbon Nanoparticles Using 3-(4,5-Dimethylthiazol-2-yl)-5-(3-Carboxymethoxyphenyl)-2-(4-Sulfophenyl)-2H-Tetrazolium (MTS) Cell Viability Assay
  Amanda M. Schrand, Jonathan B. Lin, and Saber M. Hussain
• 33. Luciferase Reporter System for Studying the Effect of Nanoparticles on Gene Expression
  Min Ding, Linda Bowman, and Vincent Castranova
• 34. Single Cell Gel Electrophoresis Assay (Comet Assay) for Evaluating Nanoparticles-Induced DNA Damage in Cells
  Linda Bowman, Vincent Castranova, and Min Ding
• 35. Assessment of Toxicity of Nanoparticles Using Insects as Biological Models
  Yan Zhou, Aracely Rocha, Carlos J. Sanchez, and Hong Liang
• 36. Evaluation of Cytotoxicity of 3-Mercaptopropionic Acid-Modified Quantum Dots on Medicago sativa Cells and Tissues
  Ana Raquel Santos, Ana Sofia Miguel, Pedro Fevereiro, and Abel Oliva

Part VI: Nanoparticles Characterization

• 37. Scanning Transmission Electron Microscopy Methods for the Analysis of Nanoparticles
  Arturo Ponce, Sergio Mejía-Rosales, and Miguel José–Yacamán
• 38. Total Internal Reflection Microscopy (TIRF) for Real-Time Imaging of Nanoparticle-Cell Plasma Membrane Interaction
  Ladan Parhamifar and S. Moein Moghimi
• 39. Microscopy Imaging Methods for the Detection of Silver and Titanium Nanoparticles Within Cells
  Robert Martin Zucker and Kaitlin Marie Daniel
• 40. Detection of TiO2 Nanoparticles in Cells by Flow Cytometry
  Robert Martin Zucker and Kaitlin Marie Daniel
• 41. NanoParticle Tracking Analysis for the Multiparameter Characterization and Counting of Nanoparticle Suspensions
  Matthew Wright
• 42. Relevance of Two-Dimensional Brownian Motion Dynamics in Applying Nanoparticle Tracking Analysis
  Paul Van der Meeren, Marios Kasinos, and Hans Saveyn
• 43. Nanoparticle Bridges for Studying Electrical Properties of Organic Molecules
  Klaus Leifer, Ken Welch, Syed Hassan Mujtaba Jafri, and Tobias Blom