Vascular Biology Protocols

Description

• Includes new updates on traditional methods
• Provides various methodologies for gene therapy and gene-knockout
• Highly specific, easy to follow step-by-step laboratory protocols

Over the past decades, the pathogenesis, diagnosis, treatment and prevention of cardiovascular diseases have been benefited significantly from intensive research activities. In order to provide a comprehensive "manual" in a field that has become as broad and deep as cardiovascular medicine, Vascular Biology Protocols covers a wide spectrum of in vivo and in vitro techniques encompassing biochemical, pharmacological and molecular biology disciplines which are currently used to assess vascular disease progression. Each chapter included in this volume focuses on a specific vascular biology technique and describes various applications as well as caveats of these techniques. The protocols included here are described in detail, allowing beginners with little experience in the field of vascular biology to embark on new research projects.

Comprehensive and easy-to-use, Vascular Biology Protocols will prove valuable for all those who wish to broaden their knowledge of cardiovascular research tools.

Reviews:

"This book of detailed protocols for researchers in the field of vascular biology is particularly relevant for scientists using animal and cell culture models. … Researchers new to the techniques commonly used to study cardiovascular disease, including clinician scientists, basic research scientists, graduate students, or residents are the intended audience. … This would be a valuable addition to any laboratory doing research in vascular biology."
(Vera P. Shively, Doody’s Review Service, December, 2008)

Table of Contents

• Preface
• Contributors
• Rat Carotid Artery Balloon Injury Model
  David A. Tulis
• Histological and Morphometric Analyses for Rat Carotid Artery Balloon Injury
  David A. Tulis
• Plaque Rupture Model in Mice
  Takeshi Sasaki, Kae Nakamura and Masafumi Kuzuya
• Immunostaining of Mouse Atherosclerotic Lesions
  Hong Lu, Debra L. Rateri and Alan Daugherty
• Surgical Animal Model of Ventricular Hypertrophy
  Giuseppe Marano and Alberto U. Ferrari
• Animal Models of Hypertension
  Brett M. Mitchell, Thomas Wallerath and Ulrich Förstermann
• Rat Models of Cardiac Insulin Resistance
  Sanjoy Ghosh, Brian Rodrigues and Jun Ren
• The Isolated, Perfused Pseudo-Working Heart Model
  Gary J. Grover and Rajni Singh
• Altering and Analyzing Glucose Metabolism in Perfused Hearts of Transgenic Mice
  Rajakumar V. Donthi and Paul N. Epstein
• Methods in Evaluation of Cardiovascular Renin Angiotensin Aldosterone Activation and Oxidative Stress
  Camila Manrique, Guido Lastra, Javad Habibi, Yongzhong Wei, E. Matthew Morris, Craig S. Stump and James R. Sowers
• Detection and Quantification of Apoptosis in the Vasculature
  Wendy A. Boivin and David J. Granville
• Endothelial Progenitor Cells and Mesenchymal Stem Cell Isolation, Characterization and Viral Transduction
  Keith R. Brunt, Sean RR Hall, Christopher A. Ward and Luis G. Melo
• Assessment of Endothelial Damage/Dysfunction: A Focus on Circulating Endothelial Cells
  Christopher J Boos, Andrew D. Blann and Gregory Y. H. Lip
• Evaluation of Endothelial Function by In-Vivo Microscopy
  Rosario Scalia
• Haptotaxis of Endothelial Cell Migration under Flow
  Steve Hsu, Rahul Thakar, and Song Li
• Isolation Culture and Functional Analysis of Adult Mouse Cardiomyocytes
  Ronglih Liao and Mohit Jain
• Mechanical Measurement of Contractile Function of Isolated Ventricular Myocytes
  Loren E. Wold and Jun Ren
• Studying Ischemia and Reperfusion in Isolated Neonatal Rat Ventricular Myocytes Using Coverslip Hypoxia
  Kelly R. Pitts and Christopher F. Toombs
• Isolation and Functional Studies of Rat Aortic Smooth Muscle Cells
  Nair Sreejayan and Xiaoping Yang
• Detection of Reactive Oxygen Species and Nitric Oxide in Vascular Cells and Tissues: Comparison of Sensitivity and Specificity.
  Hua Cai, Sergey Dikalov, Kathy K. Griendling and David G. Harrison
• Assessment of Protein Glycoxidation in Ventricular Tissues
  Shi-Yan Li and Jun Ren
• Assessment of PI-3 kinase and Akt in Ischemic Heart Diseases in Diabetes
  Takashi Matsui and Amy J. Davidoff
• Two-dimensional Gel Electrophoresis in Platelet Proteomics Research
  Ángel García-Alonso
• Stem Cell Therapy in the Heart and Vasculature
  Loren E. Wold, Wangde Dai, Joan S. Dow and Robert A. Kloner