Apoptosis and Cancer: Methods and Protocols

About this book

Our understanding of the apoptotic pathway and its regulation has improved significantly in the last 10 years. However, as our knowledge of the intricate intracellular events increase, we realize the complexity of the regulatory mechanisms that control it. As such, Apoptosis and Cancer describes the performance of contemporary techniques for studying the biology of apoptosis and its role in cancer. This book is a collaboration between academics- and industry-based scientists. The chapters have the technical development characteristic of an academic environment and the standardized system necessary for translating a product to general use, as provided by the industry. Thus, the protocols described within this groundbreaking new volume will aid both an academic laboratory interested in further characterizing the mechanism of apoptosis and an industry laboratory interested on identifying new target molecules or screening for new compounds with potential clinical use. The authors – all leaders in the field – have created a comprehensive text that covers the newest available techniques as well as conventional basic techniques. Apoptosis and Cancer will undoubtedly provide the necessary resources to everyone interested in the area of apoptosis.

Written for:

Cancer researchers, molecular and cellular biologists, biochemists

Keywords:

• Cancer research
• Flow cytometry
• New target molecules

Table of contents

Editors: Gil Mor MD, PhD Department of Obstetrics, Gynecology and Reproductive Science Yale University School of Medicine and Ayesha Alvero MD Department of Obstetrics, Gynecology and Reproductive Science Yale University School of Medicine

Chapter 1 (review) Modulation of Apoptosis to Reverse Chemoresistance - Gil Mor, Michele K. Montagna and Ayesha B. Alvero

Chapter 2. Caspase-3 Activation is a Critical Determinant of Genotoxic Stress-Induced Apoptosis - Suparna Mazumder1, Dragos Plesca1,3, and Alexandru Almasan1,2,

Chapter 3. Flow cytometry enumeration of apoptotic cancer cells by apoptotic rate *David Diaz, BSc *Alfredo Prieto BSc, PhD, *, Eduardo Reyes, BSc, PhD, *Hugo Barcenilla, BSc, *Jorge Monserrat, BSc, PhD, *, Melchor Alvarez-Mon, MD, PhD.

Chapter 4 Detection of cancer-related proteins in fresh-frozen ovarian cancer samples using Laser capture microdissection
Dan-Arin Silasi, Ayesha B. Alvero, Jechiel Mor, Rui Chen, Han-Hsuan Fu, Michele K. Montagna and Gil Mor

Chapter 5 Flow cytometric detection of activated caspase-3 - Richard Fox1 and Martine Aubert2

Chapter 6 The ChemoFx® Assay: An Ex Vivo Chemo-Sensitivity and Resistance Assay for Predicting Patient Response to Cancer Chemotherapy - Stacey L. Brower, Ph.D., Jeffrey E. Fensterer, Jason E. Bush, Ph.D., MBA

Chapter 7 Correlation of caspase activity and in vitro chemo-response in epithelial ovarian cancer cell lines - Ayesha B. Alvero, Michele K. Montagna, and Gil Mor

Chapter 8 Assessing Expression of Apoptotic Markers Using Large Cohort Tissue Microarrays - Elah Pick, Mary M. McCarthy and Harriet M. Kluger

Chapter 9 Mitochondria potential, Bax "activation" and programmed cell death. - C. Michael Knudson and Nicholas M. Brown

Chapter 10 In Vitro and In Vivo Apoptosis Detection Using Membrane Permeant, Fluorescent Labeled Inhibitors of Caspases - Brian W. Lee*, Michael R. Olin, Gary L. Johnson, and Robert J. Griffin

Chapter 11 Caspase Activity Assays - Andrew L. Niles, Richard A. Moravec, Terry L. Riss

Chapter 12 Multiplex Caspase Activity and Cytotoxicity Assays - Andrew L. Niles, Richard A. Moravec, Terry L. Riss

Chapter 13 Homogeneous, Bioluminescent Proteasome Assays - Martha A. O'Brien, Richard A. Moravec, Terry Riss, and Robert F. Bulleit

Chapter 14 A simple method for profiling miRNA expression - Jia-Wang Wang, PhD*, Jin Q. Cheng, Ph.D., M.D*.

Chapter 15 Apoptotic Caspase Activation and Activity - Jean-Berard Denault and Guy S. Salvesen

Chapter 16 Biochemical Analysis of the native TRAIL Death-inducing Signalling Complex (DISC) - Henning Walczak and Tobias L. Haas

Chapter 17 Laser Microdissection Sample Preparation for RNA Analyses - Christopher J. Vega