Core Principles in Cellular Therapy

Four chapters that specifically address cellular therapy have been extracted from the 16th edition of the AABB Technical Manual and reprinted in this handy separate volume. Written by named experts and peer-reviewed by the AABB Cellular Therapy Committee, these chapters form a key resource for a distinct readership in laboratories that typically have not used the Technical Manual.

Chapters cover:

• Collection and processing of marrow.
• HPCs collected by apheresis.
• Umbilical cord blood.
• Transfusion support for HPC recipients.

Table of Contents

Preface

1. Collecting and Processing Marrow Products for Transplantation
Janice Davis-Sproul, MAS, MT(ASCP)SBB; N. Rebecca Haley, MD; and John D. McMannis, PhD
Diseases Treated with Hematopoietic Transplantation
Autologous Hematopoietic Cell Transplantation
Allogeneic Hematopoietic Cell Transplantation
Unrelated Donor Transplantation
Histocompatibility
Graft-vs-Host Disease
Autologous Donors
Allogeneic Donors
Marrow Harvest
Collection Targets
Clinical Considerations
CD34 Antigen
Processing of HPC-M Products
ABO Incompatibilities
Short- and Long-Term Storage
Transportation and Shipping
Thawing and Infusion
Evaluation and Quality Control of Hematopoietic Products
Regulations
Standards
References
Appendix 1-1. Infusing Cryopreserved Hematopoietic Cells

2. Hematopoietic Progenitor Cells Collected by Apheresis
Thomas Lane, MD, and John D. McMannis, PhD
The Biology of HPC Mobilization
Clinical Mobilization Regimens
Clinical Use of HPC-A
Selection of an HPC-A Graft vs an HPC-M Graft
Clinical Transplantation Outcomes of HPC-A vs HPC-M Grafts
Selection of an HPC-A or HPC-M Graft vs an HPC-C Graft
References

3. Umbilical Cord Blood
David H. McKenna, MD; Diane M. Kadidlo, MT(ASCP)SBB; and Jeffrey McCullough, MD
Donor-Related Issues
Collection
Processing
Tests after Thawing and Stability Testing
Shipment and Storage
Receipt of UCB for Transplantation
Thawing and Washing of UCB
Infusion of UCB
Regulations and Standards
Novel UCB-Derived Cellular Therapies
References
Appendix 3-1. Processing Umbilical Cord Blood

4. Transfusion Support for Hematopoietic Transplant Recipients
Zbigniew M. Szczepiorkowski, MD, PhD, FCAP
ABO and Red Cell Antigen Incompatible Transplantation
Transfusion Support for ABO-Incompatible Transplant Recipients
HSCT Recipients with HLA and/or Platelet Antibodies
Neutropenic Patients with Infection Unresponsive to Antimicrobial Therapy
Utilization of Blood Components by HSCT Recipients
Special Processing of Blood Components for the Recipients of HSCT
Other Practical Considerations
References

Index