Sperm Chromatin. Biological and Clinical Applications in Male Infertility and Assisted Reproduction

DESCRIPTION

Sperm DNA damage is common and has been associated with reduced rates of conception, impaired embryonic development and increased risk of miscarriage. Although the exact causes of sperm DNA damage are unknown, it is clear that infertile men possess substantially higher levels of sperm DNA damage than do fertile men. Written by leading, internationally renowned clinicians and basic scientists with expertise in sperm DNA, Sperm Chromatin: Biological and Clinical Applications in Male Infertility and Assisted Reproduction provides readers with a thoughtful and comprehensive review of the biological and clinical significance of sperm DNA damage. The work covers the fundamental principles of sperm chromatin architecture and function, the proposed modes of DNA damage and repair, the tests of sperm DNA damage, the clinical aspects of DNA damage and the impact of DNA damage on reproductive outcome. Unlike any other title on the topic, Sperm Chromatin: Biological and Clinical Applications in Male Infertility and Assisted Reproduction is an invaluable addition to the literature and will serve as an indispensable resource for basic scientists with an interest in sperm biology and for urologists, gynecologists, reproductive endocrinologists, and embryologists working in the field of infertility.

TABLE OF CONTENTS

I. Human Sperm Chromatin: Structure and Function

• 1. Sperm Chromatin: An Overview
  Rodney Balhorn
• 2. Spermatogenesis: An Overview
  Rakesh Sharma and Ashok Agarwal
• 3. Sperm Nucleoproteins
  Rafael Oliva and Judit Castillo
• 4. The Relationship Between Chromatin Structure and DNA Damage in Mammalian Spermatozoa
  Kenneth Dominguez, Chris D. R. Arca, and W. Steven Ward
• 5. Chromosome Positioning in Spermatozoa
• Andrei Zalensky, Olga Mudrak, and Irina Zalenskaya
• 6. Sperm Mitochondrial DNA
  Justin C. St. John and Bianca St. John
• 7. The Sperm Epigenome
  Donovan Chan and Jacquetta Trasler
• 8. RNA Expression in Germ Cells During Spermatogenesis (Germ Cell Transcriptome)
  Tin-Lap Lee, Albert Hoi-Hung Cheung, Owen M. Rennert, and Wai-Yee Chan

II. Laboratory Evaluation of Sperm Chromatin

• 9. Sperm Chromatin Structure Assay (SCSA®): 30 Years’ Experience with the SCSA®
  Donald P. Evenson
• 10. Sperm Chromatin Dispersion Test: Technical Aspects and Clinical Applications
  Jaime Gosálvez, Carmen López-Fernández, and José Luís Fernández
• 11. Basic and Clinical Aspects of the Sperm Chromomycin A3 Assay
  Giancarlo Manicardi, Davide Bizzaro, and Denny Sakkas
• 12. Cytochemical Tests for Sperm Chromatin Maturity
  Igor Tsarev and Juris Erenpreiss
• 13. Acridine Orange Test for Assessment of Human Sperm DNA Integrity
  Alex C. Varghese, C. Fischer–Hammadeh, and M. E. Hammadeh
• 14. Laboratory Evaluation of Sperm Chromatin: TUNEL Assay
  Rakesh Sharma and Ashok Agarwal
• 15. The Basic and Clinical Aspects of the Sperm Comet Assay
  Luke Simon and Sheena E. M. Lewis
• 16. Assays Used in the Study of Sperm Nuclear Proteins
  Timothy G. Jenkins, Benjamin R. Emery, and Douglas T. Carrell
• 17. Sperm Epigenetic Profile
  Cristina Joana Marques, Alberto Barros, and Mário Sousa
• 18. Clinical Significance of Sperm RNA
  Jean-Pierre Dadoune, Isabelle Galeraud-Denis, and Serge Carreau

III. Biological and Clinical Determinants of Sperm Chromatin Damage

• 19. Role of Oxidative Stress in the Etiology of Sperm DNA Damage
  R. John Aitken and Geoffry N. De Iuliis
• 20. Abortive Apoptosis and Sperm Chromatin Damage
  Hasan M. El-Fakahany and Denny Sakkas
• 21. Spermiogenesis in Sperm Genetic Integrity
  Marie-Chantal Grégoire, Frédéric Leduc, and Guylain Boissonneault
• 22. Male Subfertility and Sperm Chromatin Damage
  Mona Bungum, Aleksander Giwercman, and Marcello Spanò
• 23. Aging and Sperm DNA Damage
  Fábio F. Pasqualotto and Eleonora B. Pasqualotto
• 24. Cancer in Males: Implications for Sperm Quality, Fertility, and Progeny Outcome
  Peter Chan and Bernard Robaire
• 25. Sperm Chromatin and Environmental Factors
  Aleksander Giwercman
• 26. Effects of Male Accessory Gland Infection on Sperm Parameters
  Aldo E. Calogero, Sandro La Vignera, Rosita A. Condorelli, Rosario D’Agata, and Enzo Vicari

IV. Sperm Chromatin and Assisted Reproductive Technology Outcomes

• 27. The Impact of Sperm Processing and Cryopreservation on Sperm DNA Integrity
  Dan Yu, Luke Simon, and Sheena E. M. Lewis
• 28. Structure of Chromatin in Human Sperm Bound to Hyaluronic Acid: The Benefits of PICSI Dish Mediated Sperm Selection
  Leyla Sati and Gabor Huszar
• 29. Electrophoretic Sperm Separation
  Steven Fleming and R. John Aitken
• 30. Antioxidants and Sperm DNA Damage
  Armand Zini and Maria San Gabriel
• 31. Sperm Chromatin and ART (IUI, IVF, and ICSI) Pregnancy
• Mona Bungum
• 32. Sperm DNA Damage and Pregnancy Loss After IVF/ICSI
  Armand Zini and Jason Matthew Boman
• 33. Postnatal Effects of Sperm Chromatin Damage
  Miriam Pérez-Crespo, Raúl Fernández-González, Miguel Ángel Ramírez, Eva Pericuesta, Alexandra Calle, and Alfonso Gutiérrez-Adán
• 34. Evaluation of Chromatin and DNA Integrity in Testicular Sperm
  Armand Zini and Naif Al-Hathal

V. Protocols and Integrity Tests

• 35. The Alkaline Comet Assay
  Sheena E. M. Lewis
• 36. Sperm Chromatin Structure Assay (SCSA)
  Donald P. Evenson
• 37. Measurement of DNA Damage in Spermatozoa by TUNEL Assay
  Rakesh Sharma and Ashok Agarwal
• 38. Clinical Utility of Sperm DNA Integrity Tests
  Armand Zini