A Practical Guide to Frozen Section Technique

About this book

A Practical Guide to Frozen Section Technique offers an easy to learn approach to frozen section technique in the form of a highly illustrated handbook intended for onsite use in the laboratory. The book begins with a novel, clearly delineated, step by step approach to learning continuous motion brush technique. Emphasis is placed on recognizing and correcting artifacts during the preparation process. The book addresses all of the steps in the preparation of slides from cutting through cover-slipping. The author’s unique, original techniques for tissue embedding including face down embedding in steel well bars, frozen block cryoembedding and paper cryoembedding are detailed. Variables key to the quality of the preparation including block temperature, tissue properties and section thickness are detailed. The book also covers understanding the cryostat and basic maintenance and care.Sections covering techniques used in Mohs dermatologic surgery, and techniques used in basic animal and human research are discussed by noted experts in their field.

A Practical Guide to Frozen Section Technique will be of great value to pathologists, pathology residents in training and also experimental pathology researchers that rely upon this methodology to perform tissue analysis in research.

Written for: Practicing surgical pathologists, pathology residents in training, histotechnologists, experimental pathology researchers

Keywords:

Cytologic Preparations
Embedding Techniques
Fixation
Frozen Section
Mohs Surgery
Staining and Coverslipping

Table of contents

Chapter 1 Understanding and Maintaining the Cryostat
Philip Hyam

Chapter 2 Gross Examination of Tissues in the Frozen Section Room
Stephen R. Peters MD

Chapter 3 Embedding of Tissue for Frozen Section.
Stephen R. Peters MD

Chapter 4 Cutting the Frozen Section
Stephen R. Peters MD

Chapter 5 Variables Affecting the Cutting Properties of Tissues and the Resulting Artifacts
Stephen R Peters MD

Chapter 6 Fixation, Staining and Coverslipping of Frozen Section Slides
Stephen R Peters MD, Catharine Susan Delia HT (ASCP)

Chapter 7 Microscopic Interpretation in the Frozen Section Setting
Stephen R Peters MD

Chapter 8Frozen Section Techniques used in Mohs Micrographic Surgery
Barbara S. Beck, HT (ASCP), Stephen Peters MD

Chapter 9 Frozen Section Technique in the Animal Research Setting
Charles W. Scouten, PhD.