Molecular Detection of Human Parasitic Pathogens

Description

Traditionally, laboratory identification of parasites has relied upon various phenotypic procedures that detect their morphological, biological, and immunological features. Because these procedures tend to be time-consuming and technically demanding, molecular methods based on nucleic acid amplification technologies have been increasingly utilized for rapid, sensitive, and specific characterization of parasites.

The large number of original and modified molecular protocols that have been developed over the years creates a dilemma for those attempting to adopt the most appropriate protocol for streamlined identification and detection of human pathogenic organisms of interest. Part of a four-volume collection, Molecular Detection of Human Parasitic Pathogens provides a reliable and comprehensive resource on the molecular detection and identification of major human parasitic pathogens.

This volume contains expert contributions from international scientists involved in human parasitic pathogen research and diagnosis. Following a similar format throughout, each chapter includes:

• A brief review on the classification, biology, epidemiology, clinical features, and diagnosis of an important pathogenic parasitic genus/group
• An outline of clinical sample collection and preparation procedures and a selection of representative stepwise molecular protocols
• A discussion on further research needs relating to improved diagnoses of major human parasitic pathogens

This versatile reference on molecular detection and identification of major human parasitic pathogens is an indispensable tool for upcoming and experienced medical, veterinary, and industrial laboratory scientists engaged in parasite characterization. It is also suitable as a textbook for undergraduate and graduate students majoring in parasitology.

Features

• Written by experts in respective fields of human parasitic pathogen research and diagnosis
• Offers a comprehensive coverage of important human parasitic pathogens
• Presents an authoritative summary of biology, epidemiology, and pathogenesis of major human parasitic pathogens
• Provides an in-depth review of state-of-the-art molecular detection and identification methods for human parasitic pathogens

Table of Contents

• Introductory Remarks
  Dongyou Liu

Section I Sarcomastigophora

• Acanthamoeba
  Hélene Yera, Muriel Cornet, and Jean-Louis Bourges
• Balamuthia
  Govinda S. Visvesvara and Yvonne Qvarnstrom
• Blastocystis
  Hisao Yoshikawa
• Dientamoeba
  Damien Stark
• Entamoeba
  Damien Stark and John Ellis
• Giardia
  Yaoyu Feng and Lihua Xiao
• Leishmania
  Clotilde Marín, Silvia S. Longoni, and Manuel Sánchez-Moreno
• Naegleria
  Mineko Shibayama, Jesús Serrano-Luna, Isaac Cervantes-Sandoval, and Víctor Tsutsumi
• Pentatrichomonas
  Tania Crucitti
• Trichomonas
  Dongyou Liu, Xiangang Kong, and Changyou Xia
• Trypanosoma
  Emily R. Adams

Section II Ciliophora and Apicomplex

• Babesia
  Dongyou Liu and Xuedong Lu
• Balantidium
  Dongyou Liu
• Cryptosporidium
  Una M. Ryan and Rongchang Yang
• Cyclospora
  Dongyou Liu
• Isospora
  Chaturong Putaporntip and Somchai Jongwutiwes
• Plasmodium
  Dongyou Liu and Yi-Wei Tang
• Sarcocystis
  L. Vangeel, K. Houf, P. Geldhof, J. Vercruysse, R. Ducatelle, and K. Chiers
• Toxoplasma
  Kjetil Asbakk and Kristin Wear Prestrud

Section III Platyhelminthes: Cestoda

• Diphyllobothrium
  Barbara Wicht, Raffaele Peduzzi, Hélene Yera, and Jean Dupouy-Camet
• Dipylidium
  Dongyou Liu
• Echinococcus and Echinococcosis
  Akira Ito, Tetsuya Yanagida, Yasuhito Sako, Minoru Nakao, Kazuhiro Nakaya, Jenny Knapp, and Yuji Ishikawa
• Hymenolepis
  B.R. Mirdha and Shabeena Rehman
• Mesocestoides
  Kerry A. Padgett, Paul R. Crosbie, and Walter M. Boyce
• Spirometra
  Dongyou Liu, Jianshun Chen, and Weihuan Fang
• Taenia
  Munehiro Okamoto and Akira Ito

Section IV Platyhelminthes: Trematoda

• Clonorchis
  Heinz Mehlhorn, Boris Müller, and Jürgen Schmidt
• Dicrocoelium
  Donato Traversa, Vincenzo Lorusso, and Domenico Otranto
• Echinostomes
  Rafael Toledo and Bernard Fried
• Fasciola
  Dongyou Liu and Xing-Quan Zhu
• Fasciolopsis
  Veena Tandon, B. Roy, and P.K. Prasad
• Gastrodiscoides
  Dongyou Liu
• Haplorchis
  Urusa Thaenkham and Jitra Waikagul
• Heterophyidae
  Ron Dzikowski and Michael G. Levy
• Metagonimus
  Jae-Ran Yu and Jong-Yil Chai
• Nanophyetus
  Dongyou Liu
• Opisthorchis
  Paiboon Sithithaworn, Thewarach Laha, and Ross H. Andrews
• Paragonimus
  Hiromu Sugiyama, Takhellambam Shantikumar Singh, and Achariya Rangsiruji
• Plagiorchis
  Sun Huh
• Schistosoma and Schistosomiasis
  Antonio Muro and Luís Pérez del Villar
• Trichobilharzia
  P. Horák, F.M. Schets, L. Kolárˇová, and S.V. Brant

Section V Nematoda

• Ancylostoma
  Jaco J. Verweij
• Angiostrongylus
  Dongyou Liu
• Anisakis
  Stefano D’Amelio, Serena Cavallero, Marina Busi, Sofia Ingrosso, Elisabetta Giuffra, Sarra Farjallah, and Graça Costa
• Ascaris
  Dwight D. Bowman and Janice L. Liotta
• Baylisascaris
  Dongyou Liu
• Brugia
  Kosum Chansiri and Supatra Areekit
• Capillaria
  Dongyou Liu
• Dioctophyme
  Dongyou Liu
• Dirofilaria
  Andrea Gustinelli, Maria Letizia Fioravanti, Monica Caffara, Lorella Garagnani, and Francesco Rivasi
• Dracunculus
  Dongyou Liu
• Enterobius
  Dongyou Liu
• Gnathostoma
  Paron Dekumyoy, Tippayarat Yoonuan, and Jitra Waikagul
• Haemonchus
  Michael B. Hildreth and Aaron Harmon
• Loa
  Fousseyni S. Touré Ndouo
• Mansonella
  Ramiro Morales-Hojas
• Marshallagia
  Jesús M. Pérez and Luca Rossi
• Necator
  Jan M. Schwenkenbecher
• Oesophagostomum
  Jaco J. Verweij
• Onchocerca
  Ramiro Morales-Hojas
• Pseudoterranova and Contracaecum
  Simonetta Mattiucci, Michela Paoletti, Stephen C. Webb, and Giuseppe Nascetti
• Strongyloides
  Dongyou Liu
• Syphacia
  Sanford H. Feldman and John H. Porter
• Thelazia
  Domenico Otranto and Donato Traversa
• Toxocara
  Anna Borecka
• Trichinella
  Edoardo Pozio and Giuseppe La Rosa
• Trichostrongylus
  Tai-Soon Yong
• Trichuris
  C. Cutillas, M. de Rojas, and R. Callejón
• Wuchereria
  Dongyou Liu and Frank W. Austin

Section VI Arthropoda: Arachnida

• Argasidae (Soft Ticks)
  Dongyou Liu
• Demodex (Hair Follicle Mite)
  Dongyou Liu
• Dermanyssus, Ornithonyssus, and Trombicula (red, fowl, and harvest mites)
  Dongyou Liu
• Ixodidae (Hard Ticks)
  Dongyou Liu and Chong Yin
• Pyroglyphidae and Glycyphagidae (House Dust and Storage Mites)
  Dongyou Liu
• Sarcoptes (Itch Mite)
  Dongyou Liu

Section VII Arthropoda: Insecta

• Calliphoridae, Oestridae, and Sarcophagidae (Myiasis-Causing Flies)
  Dongyou Liu
• Cimex (Bedbug)
  Dongyou Liu
• Pediculus (Body and Head Lice)
  Dongyou Liu and Sacha Stelzer-Braid
• Phthirus (Crab Louse)
  Dongyou Liu
• Pulex, Xenopsylla, and Ctenocephalides (Human, Rat and Dog/Cat Fleas)
  Dongyou Liu
• Tunga (Jigger Flea)
  Dongyou Liu

Section VIII Arthropoda: Pentastomida

• Armillifer, Linguatula, and Porocephalus (Tongue Worms)
  Dongyou Liu