Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology

Foreword Keith Wilson and John Walker
Preface Samuel Clokie and Andreas Hofmann
Tables and resources Andreas Hofmann
1. Life science studies Samuel Clokie and Andreas Hofmann
2. Basic principles Parisa Amani and Andreas Hofmann
3. Cell culture techniques Anwar Baydoun
4. Recombinant DNA techniques and molecular cloning Ralph Rapley
5. Preparative biochemistry Samuel Clokie
6. Electrophoresis Ralph Rapley
7. Immunochemical techniques Katja Fischer
8. Flow cytometry John Grainger and Joanne Konkel
9. Radioisotope techniques Robert Slater
10. Clinical biochemistry Gillian Rumsby
11. Microscopy Stephen Paddock
12. Centrifugation Kay Ohlendieck and Stephen Harding
13. Spectroscopic techniques Anne Simon and Andreas Hofmann
14. Basic techniques probing molecular structure and interactions Anne Simon and Joanne Macdonald
15. Mass spectrometric techniques Sonja Hess and James MacRae
16. Fundamentals of bioinformatics Cinzia Cantacessi and Anna Protasio
17. Fundamentals of cheminformatics Paul Taylor
18. The Python programming language Timothy Stevens
19. Processing and visualisation of experimental data Jean-Baptiste Cazier
20. Fundamental genome sequencing and annotation Pasi Korhonen and Robin Gasser
21. Fundamentals of proteomics Sonja Hess and Michael Weiss
22. Fundamentals of metabolomics James MacRae
23. Enzymes and receptors Megan Cross and Andreas Hofmann
24. Drug discovery and development David Camp.

Bringing this best-selling textbook right up to date, the new edition uniquely integrates the theories and methods that drive the fields of biology, biotechnology and medicine, comprehensively covering both the techniques students will encounter in lab classes and those that underpin current key advances and discoveries. The contents have been updated to include both traditional and cutting-edge techniques most commonly used in current life science research. Emphasis is placed on understanding the theory behind the techniques, as well as analysis of the resulting data. New chapters cover proteomics, genomics, metabolomics, bioinformatics, as well as data analysis and visualisation. Using accessible language to describe concepts and methods, and with a wealth of new in-text worked examples to challenge students' understanding, this textbook provides an essential guide to the key techniques used in current bioscience research.