Alcamo's Laboratory Fundamentals of Microbiology

Description

This popular lab manual offers thirty-four multi-part lab exercises designed to provide students with basic training in the handling of microorganisms, while exploring microbial properties and uses. This lab manual can also be used independently of the main text.

An instructor’s manual, downloadable from the Web, accompanies the lab manual and provides principles of lab safety; research topic ideas, information on customizing laboratory programs with the manual; helpful suggestions for setting up and running each exercise; and lists of laboratory media, cultures, and special materials used in each exercise.

Applicable Courses
Ideal for a microbiology laboratory course for non-majors, allied health, and nursing students.

Table of Contents

Part 1 Basic Laboratory Techniques and Skills
Exercise 1 Culture Transfer Techniques
Exercise 2 Pure Culture Techniques

Part 2 Microscopy and Bacterial Staining Techniques
Exercise 3 The Compound Microscope
Exercise 4 Preparation of a Bacterial Smear and the Simple Stain Technique
Exercise 5 The Negative Stain Technique
Exercise 6 The Gram Stain Technique
Exercise 7 Bacterial Structures

Part 3 Viruses and Eukaryotic Microorganisms
Exercise 8 Viruses
Exercise 9 Fungi, Protozoa, and Multicellular Parasites

Part 4 The Spread and Control of Microorganisms
Exercise 10 A Simulated Epidemic
Exercise 11 The Effect of Physical Agents on Bacteria
Exercise 12 The Effect of Chemical Agents on Bacteria
Exercise 13 Evaluation of Disinfectants and Antiseptics
Exercise 14 The Effect of Antibiotics on Bacteria
Exercise 15 Microbial Growth: Analysis of a Bacterial Growth Curve

Part 5 Medical Microbiology
Exercise 16 The Genus Mycobacterium
Exercise 17 The Genus Streptococcus
Exercise 18 The Genus Neisseria
Exercise 19 The Genus Staphylococcus
Exercise 20 The Enteric Bacteria
Exercise 21 The Genus Bacillus
Exercise 22 The Genus Clostridium
Exercise 23 The Genus Lactobacillus

Part 6 Identification of a Bacterial
Exercise 24 Bacterial Structural Characteristics
Exercise 25 Bacterial Culture Characteristics
Exercise 26 Biochemical Characteristics of Bacteria

Part 7 Bacterial Genetics and Serology
Exercise 27 Bacterial Genetics: Mutations and the Ames Test
Exercise 28 Bacterial Conjugation
Exercise 29 Bacterial Transformation
Exercise 30 Serology: Agglutination Techniques and Blood Cell Identification

Part 8 Public Health and Environmental Microbiology
Exercise 31 Microbiology of Foods
Exercise 32 Microbiology of Milk and Dairy Products
Exercise 33 Microbiology of Water
Exercise 34 Microbiology of Soil

Author

Jeffrey C. Pommerville, PhD-Glendale Community College

Congratulations to Dr. Pommerville who was recently awarded the 2008 Carski Foundation Distinguished Undergraduate Teaching Award!

Jeffrey Pommerville has a Ph.D. in Cell and Organismal Biology from the University of California, Santa Barbara. After serving as a Postdoctoral Research Fellow at the University of Georgia, he was an assistant professor of biology at Texas A&M University. For the past 14 years, he has been Professor of Biology at Glendale Community College where he teaches introductory biology and microbiology. He has 20 years of research experience in cell biology and microbiology and has authored over 35 peer-reviewed papers in national and international research journals. For the past three years, he was the principal investigator for Systemic Reform In Science (SyRIS), a project funded by the National Science Foundation that was designed to improve student outcomes in science through changes in curriculum and pedagogy that align with national systemic reform initiatives. In 2003, he was awarded the Gustav Ohaus Award (College Division) for Innovations in Science Teaching from the National Science Teachers Association. Over the past three years, he has presented numerous seminars and workshops to colleges, universities, business, medical, and service organizations on understanding and responding to bioterrorism.