Brain Tumor Stem Cells. Methods And Protocols

1. Front Matter

   Pages i-xii

2. Brain Tumor Stem Cells: New Perspectives

   • Alisha Anand, Chitra Venugopal, Sheila K. Singh

   Pages 1-15

3. Isolation and Propagation of Brain Tumor Stem Cells

   • Anish Puri, Chitra Venugopal, Sheila K. Singh

   Pages 17-25

4. Embryonic Stem Cell Models of Human Brain Tumors

   • Ludivine Coudière Morrison, Nazanin Tatari, Tamra Werbowetski-Ogilvie

   Pages 27-42

5. Isolation and Culture of Human Neural Stem Cells

   • Agata M. Kieliszek, Yujin Suk, Daniel Mobilio, Chitra Venugopal, Sheila K. Singh

   Pages 43-48

6. Metabolic Assays for Brain Cancer Cells

   • Emma Martell, Harshal Senthil, Esha Kaul, Helgi Kuzmychova, Tanveer Sharif

   Pages 49-64

7. Synergy Assay for Screening Small Molecule Combinations in Brain Cancer Stem Cells

   • Benjamin Brakel, Iqra Chaudhry, Chitra Venugopal, Sheila K. Singh

   Pages 65-71

8. Extracellular Flux Assays for the Measurement of Glycolysis and Mitochondrial Respiration in Brain Cancer Cells

   • Kanwaldeep Singh, Tobias Berg

   Pages 73-109

9. O6-Methylguanine–DNA Methyltransferase (MGMT) Promoter Methylation Analysis in Glioblastoma Patients

   • Petar Miletic, Benjamin Brakel, Chitra Venugopal, Sheila K. Singh

   Pages 111-118

10. Profiling Glioma Stem Cell Dynamics via 3D-Based Cell Cycle Reporter Assays

    • Dorota Lubanska, Antonio Roye-Azar, Sami Alrashed, Alan Cieslukowski, Mohamed A. R. Soliman, Ana C. deCarvalho et al.

    Pages 119-134

11. Proximity Ligation Assay

    • Shan Grewal, Shawn C. Chafe, Chitra Venugopal, Sheila K. Singh

    Pages 135-140

12. Efficient Migration Assay for Brain Tumor Stem Cells

    • Daniel Mobilio, Agata M. Kieliszek, Chitra Venugopal, Sheila K. Singh, Shawn C. Chafe

    Pages 141-148

13. Patient-Derived Glioblastoma Explants Empower Rapid and Personalized Drug Assessment: Harnessing the Potential of 3D Perfusion Bioreactors in Glioblastoma Drug Discovery

    • Nazanin Tatari, Manuele G. Muraro, Hayget Mohamed Seid Hedad, Gregor Hutter

    Pages 149-161

14. Chimeric Antigen Receptor T Cell Production for Brain Cancers

    • William T. Maich, Benjamin Brakel, Chitra Venugopal, Sheila K. Singh

    Pages 163-171

15. CRISPR-Cas9-Guided Genetic Manipulation of Patient-Derived Brain Tumor Cells

    • Yujin Suk, Erika Apel, Stefan Custers, Petar Miletic, Kui Zhai, Chirayu Chokshi et al.

    Pages 173-184

16. Unmasking Stemness and Immune Cell Markers in Brain Tumors with Immunohistochemistry

    • Jack Q. Lu, Kui Zhai, Agata M. Kieliszek, Chitra Venugopal, Sheila K. Singh

    Pages 185-191

17. In Vitro Models to Compare the Duration of Action of New Therapeutic Scaffolds and Drug Eluting Medical Devices

    • Kevin Wai, Ryan G. Wylie

    Pages 193-206

18. Cleavage Under Targets & Release Using Nuclease (CUT&RUN) Protocols for the Study of Genetic and Epigenetic Regulation in Cancer

    • Stefan Custers, Erika Apel, Yujin Suk, Chitra Venugopal, Sheila K. Singh, Etienne Danis

    Pages 207-225

19. A Toolkit for Single-Nucleus Characterization of Glioblastoma

    • Cole C. Nickason, Vanshika Khaitan, Connor Clark-Baba, Alberto G. Torrez, Mikhail Y. Salnikov, Kabir Siraj et al.

    Pages 227-237

20. Using GlioVis to Explore, Analyze, and Visualize Brain Tumor Multi-Omics Datasets

    • Patrick Ang, Chitra Venugopal, Sheila K. Singh, Laura Escudero

    Pages 239-256

     

    1. Modeling Glioma Stem Cell-Mediated Tumorigenesis Using Zebrafish Patient-Derived Xenograft Systems

       • Hema Priya Mahendran, Alan Cieslukowski, Dorota Lubanska, Nicholas Philbin, Keith F. Stringer, Philip Habashy et al.

       Pages 257-277

    2. An In Vivo Model of Recurrent Glioblastoma

       • Muhammad Vaseem Shaikh, Benjamin Brakel, Alisha Anand, Chitra Venugopal, Sheila K. Singh

       Pages 279-286

    3. Bioluminescent Imaging of Patient-Derived Brain Tumors in Live Mice

       • Dillon McKenna, Chitra Venugopal, Sheila K. Singh

       Pages 287-300

    4. Growth Dynamics of Brain Tumor Through the Lens of MT-Weighted MRI

       • Manoj Singh, Chitra Venugopal, Sheila K. Singh

       Pages 301-308

    5. Correction to: Extracellular Flux Assays for the Measurement of Glycolysis and Mitochondrial Respiration in Brain Cancer Cells

       • Kanwaldeep Singh, Tobias Berg

       Pages C1-C1

    6. Back Matter

       Pages 309-311

This updated volume explores the technically challenging study of brain tumor stem cells (BTSCs) with their unique capacity to self-renew, proliferate, and initiate tumor formation. The book collects up-to-date methodologies to isolate and propagate BTSCs and to study them using various cutting-edge techniques, in service of bringing us closer to translating the discoveries made from these cells into new therapeutic options for brain cancer patients. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Brain Tumor Stem Cells: Methods and Protocols, Second Edition provides a comprehensive understanding of the skills and techniques needed to unlock data from this most informative subset of cells.