Neural Stem Cells

1. Front Matter

   Pages i-xvi

    

2. Flow Cytometry-Based Protocols for the Mouse Subependymal Neurogenic Niche Phenotyping

   • Pere Duart-Abadia, Pau García-Bolufer, Laura Blasco-Chamarro, Tomás Viuda, Jose Manuel Morante-Redolat, Germán Belenguer

   Pages 1-19

3. Isolation of Adult Hippocampal Neural Stem Cells

   • Soraya Martín-Suárez

   Pages 21-34

4. Low-Density Primary Cell Culture of Postnatal Murine Cerebellar Progenitors In Vitro

   • Celia Llorente-Sáez, Julia Serrano-López, Esmerilda G. Delicado, Raquel Pérez-Sen, Rosa Gómez-Villafuertes, Felipe Ortega

   Pages 35-46

5. Isolation of Oligodendrocyte Precursor Cells with Immunomagnetic Beads for Their Better Viability In Vitro and In Vivo

   • Paula Gómez-Martín, Alba Macías-Castellano, Beatriz Fernández-Gómez, Estefanía Sánchez-Jiménez, Miguel Marchena, Fernando de Castro

   Pages 47-57

6. Nonhematopoietic Stem Cell Identification and Sorting in Adult Retina

   • Ana Flores, Laura Fernández-Sánchez, Oksana Kutsyr, Alberto Yáñez, María Luisa Gil, Daniel Gozalbo et al.

   Pages 59-65

7. Neurospheres from Ovarian Cortical Cells in Mammals: Generation In Vitro and Neurosphere Assay

   • Alfredo González-Gil, Rosa Ana Picazo

   Pages 67-87

8. Detection of Monocytic Myeloid-Derived Suppressor Cells in the Human Brain and Blood

   • María Cristina Ortega, Virginia Vila-del Sol, Isabel Machín-Díaz, Diego Clemente

   Pages 89-109

9. Cell-Type-Specific Protein Metabolic Labeling and Identification Using the Methionine Subrogate ANL in Cells Expressing a Mutant Methionyl-tRNA Synthetase

   • Rodrigo Alvarez-Pardo, Ella Doron-Mandel, Hector Albert-Gascó, Cristina Olmedo Salinas, Marko Jovanovic, Beatriz Alvarez-Castelao

   Pages 111-126

10. Experimental Neurogenesis in the Embryos of the Gecko Paroedura picta

    • Sara Jiménez, Rodrigo Senovilla-Ganzo, Tatiana Gallego-Flores, Erise Pérez-Pascual, Aitor Ordeñana-Manso, Raquel Rayo-Morales et al.

    Pages 127-145

11. Genetic Tracing of Progenitors from Embryo to Postnatal Brain

    • Ana Cristina Ojalvo-Sanz, María Figueres-Oñate, Sonsoles Barriola, Laura López-Mascaraque

    Pages 147-160

12. Tracking Neural Migration in Dentate Gyrus Using Thymidine Analogs

    • Álvaro Sebastián-Serrano, Paloma Aivar, Lucia Soria-Tobar, Laura Roman-Valero, Sofía Inés Martínez-Centeno, Miguel Díaz-Hernández

    Pages 161-169

13. Electroporation Techniques to Target Subependymal Zone Neurogenic Niche

    • Andrzej W. Cwetsch, Cristina Gil-Sanz

    Pages 171-182

14. In Vitro and In Vivo Direct Reprogramming of Astrocytes to Induced-Neurons

    • Elsa Papadimitriou, Paraskevi N. Koutsoudaki, Irini Thanou, Dimitra Thomaidou

    Pages 183-198

15. Retrovirus-Mediated Reprogramming of Endogenous Hippocampal Glia into GABAergic Induced Neurons

    • Marie d’Orange, Célia Lentini, Christophe Heinrich

    Pages 199-219

16. Gene Expression Manipulation Via Acute Electroporation in Human Brain Organoids

    • Athanasia Rapti, Christina Kyrousi

    Pages 221-232

17. Targeted Human Editing Using CRISPR Technology in Neural Stem Cells

    • Alvaro Gutierrez-Uzquiza, Paloma Bragado

    Pages 233-243

18. Patch-Clamp Recordings in Isolated Neural Cells, Acute Tissue Slices, and Brain Rosettes

    • Marina Arribas-Blázquez, Celia Llorente-Sáez, Luis A. Olivos-Oré, Antonio R. Artalejo

    Pages 245-262

19. Acute Slice Patch-Clamp Recordings to Characterize Neural Populations in the Hippocampal Subgranular Zone

    • Raquel Bajo-Grañeras, Alberto Samuel Suárez-Pinilla, Magdalena Torres, Ricardo Martín

    Pages 263-274

20. Intracellular Calcium Recording and Immunostaining of Single Neural Populations In Vitro

    • Julia Serrano-López, Celia Llorente-Sáez, Raquel Pérez-Sen, Esmerilda G. Delicado, Felipe Ortega, Rosa Gómez-Villafuertes

    Pages 275-287

     

    1. Cell Population Analyses of Neurospheres Generated In Vitro from Ovarian Cortical Cells in Mammals: Characterization by Immunohistochemistry and Flow Cytometry

       • Alfredo González-Gil, Belén Sánchez-Maldonado, Ángeles Sánchez Pérez, Pedro José Aranda, Rosa Ana Picazo

       Pages 289-307

    2. Reference-Based Gene Expression Analysis Using Galaxy Server

       • Sara González-Bodí, Jaime Huerta-Cepas

       Pages 309-338

    3. Development of Novel Neuroprotective Agents Targeting GPCR Using in Vitro Assays

       • María Gómez-Cañas, Carmen Rodríguez-Cueto, Javier Fernández-Ruiz, Eva de Lago

       Pages 339-366

    4. Picrotoxin-Induced Epileptogenic Hippocampal Organotypic Slice Cultures (hOTCs)

       • Ane Rodríguez-Bodero, Paolo Bonifazi, Jan Tønnesen, Juan Manuel Encinas-Pérez

       Pages 367-388

    5. Back Matter

       Pages 389-391

This detailed volume explores techniques for the study of neurogenic niches and neural lineage progression, specifically addressing major methodological challenges in studying neural stem cells (NSCs) and related cell populations, such as astrocytes, oligodendrocytes, and neurons. The chapters are organized to offer a comprehensive technical foundation, focusing on isolation and identification, monitoring, manipulation, analysis, and the exploration of potential therapeutic applications of NSCs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Neural Stem Cells: Methods and Protocols serves as an ideal guide for the scientific community and a broader audience interested in understanding neurogenic niches and the progression of neural lineages.