Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies

1. Flow cytometry in clinical haematopathology: basic principles and data analysis of multiparameter data sets Francis Lacombe and Marie-Christine Béné
2. Antigens Marie-Christine Béné and Anna Porwit
3. Flow cytometry of normal blood, bone marrow and lymphatic tissue Anna Porwit and Marie-Christine Béné
4. Reactive conditions and other diseases where flow cytometric findings may mimic haematological malignancies Marie-Christine Béné, Wolfgang Kern and Anna Porwit
5. Examples of immunophenotypic features in various categories of acute leukaemia Marie-Christine Béné and Anna Porwit
6. Acute lymphoid leukaemia (all) and minimal/measurable residual disease in all Barbara Buldini, Elena Varotto and Andrea Zangrando
7. Immunophenotyping of mature b-cell lymphomas Olof Axler and Anna Porwit
8. Plasma cell myeloma and related disorders Anna Porwit and Marie Christine Béné
9. Mature t-cell neoplasms and natural killer-cell malignancies Anne Tierens
10. Flow cytometric diagnosis of classic hodgkin lymphoma in lymph nodes Lori Soma and Jonathan R. Fromm
11. Measurable residual disease in acute myeloid leukaemia Jacqueline Cloos, Angèle Kelder, Dave de Leeuw and Costa Bachas
12. Ambiguous lineage and mixed phenotype acute leukaemia Anna Porwit and Marie-Christine Béné
13. Flow cytometry in myelodysplastic syndromes Theresia M. Westers and Arjan A. van de Loosdrecht
14. Future applications of flow cytometry and related techniques Marie Christine Béné, Anna Porwit and Francis Lacombe.

Master the implementation of flow cytometry techniques in diagnosing complex hematologic diseases and malignancies in patients with this comprehensive and practical guide. Featuring updated recommendations on pre-analytical steps, instrument settings and panel construction, this manual offers invaluable support for those diagnosing, treating and researching hematologic malignancies. Written by leading experts in the field, this book puts flow cytometry into everyday context. Through engaging with differential diagnoses, the manual offers an accurate description of specific features of malignancies and mimicking conditions. It also places flow cytometry in the scope of integrated diagnosis according to WHO and ICC classifications. Fully updated throughout, this second edition provides new literature and concepts, and presents new AI-based analytical methods. Richly illustrated and in full colour, this book is an ideal resource for those working in flow cytometry laboratories.

• Provides practical information on flow cytometry features of hematological malignancies, to apply in everyday diagnostics work-up
• Can be used as reference book in any lab dealing with hematological diagnoses
• Richly illustrated in full colour throughout

Anna Porwit, Lunds Universitet, Sweden
Anna Porwit MD PhD is Professor Emerita at the Division of Pathology, Lund University, Sweden. She has also held professor positions at the Karolinska Institute, Stockholm, Sweden, and at the University of Toronto, ON, Canada. She is a co-author of over 200 original papers and has contributed to the 'WHO Classification of Haematopoietic Malignancies' (2008 and 2016).

Marie Christine Béné, Université de Nantes, France
Marie Christine Béné is Professor Emerita of Hematology, former Professor of Immunology, and lastly head of the Hematology Biology Department in Nantes University Hospital. She has been largely involved in flow cytometry applications in diagnosis and follow-up. She has co-authored over 500 publications, has contributed to the 'WHO Classification of Haematopoietic Malignancies' (2008 and 2016) and leads the European LeukemiaNet WP10 on morphology and flow cytometry.