Pcr Primer. a Laboratory Manual

This second edition of a much - praised and widely used manual has been entirely revised and updated. Each technique is presented with extensive background information, advice, and troubleshooting. All current applications of PCR are covered, in protocols that have the hallmark reliability of the previous edition.

Contents:

Section 1. Introduction to PCR
Section 2. Sample Preparation
Section 3. RT-PCR Methods
Section 4. Detection of PCR Products: Specialized Applications
Section 5. Analysis of Differential Gene Expression by PCR
Section 6. Cloning by PCR
Section 7. Cloning of PCR Products
Section 8. Mutagenesis by PCR
Appendices