This is the textbook and reference resource that instructors, students, and researchers in biomedical optics have been waiting for. Comprehensive and up to date, it covers a broad range of areas in biomedical optics, from light interactions at the single-photon and single-biomolecule levels, to the diffusion regime of light propagation in tissue. Subjects covered include spectroscopic techniques (fluorescence, Raman, infrared, near-infrared, and elastic scattering), imaging techniques (diffuse optical tomography, photoacoustic imaging, several forms of modern microscopy, and optical coherence tomography), and laser-tissue interactions, including optical tweezers. Topics are developed from the fundamental principles of physical science, with intuitive explanations, while rigorous mathematical formalisms of theoretical treatments are also provided. For each technique, descriptions of relevant instrumentation and examples of biomedical applications are outlined, and each chapter benefits from references and suggested resources for further reading, and exercise problems with answers to selected problems.
- Takes a quantitative approach that is actionable in a research setting
- Includes practical end-of-chapter problems with solutions to selected problems
- Brings together a broad range of topics in biomedical optics from spectroscopic techniques, imaging techniques, through to laser-tissue interactions, all at various length scales
- Solutions to selected problems are available inside the book, with the remainder available on the website
2. Overview of tissue optical properties
3. Introduction to biomedical statistics for diagnostic applications
4. General concepts of tissue spectroscopy and instrumentation
5. Autofluorescence spectroscopy and reporter fluorescence
6. Raman and infrared spectroscopy of vibrational modes
7. Elastic and quasi-elastic scattering from cells and small structures
8. Diffuse reflectance spectroscopy at small source-detector separations
9. Transport theory and the diffusion equation
10. Continuous-wave methods for tissue spectroscopy
11. Time-domain methods for tissue spectroscopy in the diffusion regime
12. Frequency-domain methods for tissue spectroscopy in the diffusion regime
13. Instrumentation and experimental methods for diffuse tissue spectroscopy
14. Diffuse optical imaging and tomography
15. In vivo applications of diffuse optical spectroscopy and imaging
16. Combining light and ultrasound: acousto-optics and opto-acoustics
17. Modern optical microscopy for biomedical applications
18. Optical coherence tomography
19. Optical tweezers and laser-tissue interactions.
Irving J. Bigio, Boston University
Irving Bigio is Professor of Biomedical Engineering and Electrical Engineering at Boston University. His research activities address the interactions of light with cellular and tissue structures on the microscopic and mesoscopic scales. He pioneered methods of elastic scattering spectroscopy and has developed practical diagnostic and sensing applications that have been demonstrated in large clinical studies. He has co-authored over 200 scientific publications and is an inventor on nine patents.
Sergio Fantini, Tufts University, Massachusetts
Sergio Fantini is Professor of Biomedical Engineering at Tufts University. His research interests in biomedical optics are in the area of diffuse spectroscopy and imaging of biological tissue. He has contributed to the development of quantitative frequency-domain methods for absolute tissue oximetry, spectral imaging approaches to optical mammography, and the assessment of cerebral hemodynamics in the human brain. He has co-authored about 200 scientific publications and is an inventor on ten patents.