• Covers a broad range of host organisms, from E. coli to mammals • Focuses on the underlying principles thereby equipping the reader to devise appropriate strategies for novel situations and for use in their own research • Contains example scenarios, which allow the reader to test their understanding of the material and apply it to ‘real-life’ research situations
Preface; 1. The tools for the job; 2. Polymerase chain reaction; 3. Simple cloning; 4. Other vector systems for E. Coli; 5. Making libraries; 6. Screening libraries; 7. Modification and mutagenesis; 8. Use of cloned DNA; 9. Using other organisms; 10. Examples.