Applications of Microdialysis in Pharmaceutical Science

DESCRIPTION

Discover new and emerging applications for microdialysis in drug evaluation.

Microdialysis is a highly valuable sampling tool that can be used in vivo to measure free, unbound analyte concentrations located in interstitial and extracellular spaces. This book explores the full range of clinical applications for microdialysis, focusing on its use in different organ and tissue systems for pharmacokinetic and pharmacodynamic studies. Readers gain a full understanding of the underlying science of microdialysis, current techniques and practices, as well as its many applications in pharmaceutical research.

Applications of Microdialysis in Pharmaceutical Science starts with an introduction to basic principles and then covers analytical considerations, pharmacodynamic and pharmacokinetic studies, clinical aspects, and special applications. Topics include:

• Role of microdialysis in drug development, including crucial sampling considerations and applications for nervous system diseases
• Continuous measurement of glucose concentrations in diabetics
• Applications for clinical evaluation and basic research on organ systems, including monitoring exogenous and endogenous compounds in the lungs
• Pharmacokinetic and pharmacodynamic evaluation of anticancer drugs
• Comparison of microdialysis with imaging approaches to evaluate in vivo drug distribution
• Special applications of microdialysis in studies of cell culture assays, drug-drug interactions, and environmental monitoring

Throughout the book, readers will find simple models that clarify complex concepts and easy-to-follow examples that guide them through key applications in pharmaceutical research. In short, this book enables pharmaceutical researchers to take full advantage of microdialysis techniques for the preclinical and clinical evaluation of drugs and much more.

TABLE OF CONTENTS

1. Introduction to Applications of Microdialysis in Pharmaceutical Sciences.
2. Microdialysis in Drug Discovery.
   1. Introduction.
   2. Phases of Drug Development.
   3. Role of Biomarkers in Drug Development.
   4. Role of Pharmacokinetic-Pharmacodynamic Modeling in Drug Development.
   5. Role of Microdialysis in Drug Development.
   6. Current Applications and Perspective of Microdialysis Sampling for Drug Development of Specific Therapeutic Groups.
   7. Regulary Aspects of Microdialysis Sampling in Drug Development.
   8. Conclusions.
3. Analytical Considerations for Microdialysis Sampling.
   1. Introduction.
   2. Analytical Methodologies.
4. Monitoring Dopamine in the mesocorticolimbic and nigrostriatal systems by microdialysis: relevance for mood disorders and Parkinson's disease.
   1. Introduction.
   2. Pathophysiology of Serotonin-Dopamine Interaction: Implication for Mood Disorders.
   3. Dopamine Depletion in the Nigrostriatal System: Parkinson’S Disease.
   4. Conclusions.
5. Monitoring Neurotransmitter Amino Acids by Microdialysis: Pharmacodynamic Applications.
   1. Introduction.
   2. Monitoring neurotransmitter amino acids by microdialysis.
   3. Basic Research on receptors.
   4. Psychostimulants and addictive drugs.
   5. Analgesia.
   6. Ischemia/ anoxia.
   7. Conclusion and perspectives.
6. Microdialysis as a tool to unravel neurobiological mechanisms of seizures and antiepileptic drug action.
   1. Introduction.
   2. Microdialysis to characterise the seizure-related neurobiological and metabolic changes in animal models and in humans.
   3. Microdialysis as chemoconvulsant delivery tool in animal seizure models.
   4. Microdialysis as an elegant tool to elucidate mechanisms of electrical brain stimulation and neuronal circuits involved in the generation and control of seizures.
   5. Microdialysis to unravel the mechanisms of action of established antiepileptic drugs and new therapeutic strategies.
   6. Microdialysis studies in search for mechanisms of adverse effects of clinically used drugs, drugs of abuse and toxins.
   7. Combination of microdialysis with other complementary neurotechniques to unravel mechanisms of seizures and epilepsy.
   8. The advantage of microdialysis to sample biophase levels of antiepileptic drugs and to monitor central neurotransmitters as pharmacodynamic markers for their anticonvulsant activity.
   9. Microdialysis as a tool to study relationships between epilepsy and its comorbidities.
7. Microdialysis in lung: Monitoring of exogenous and endogenous compounds.
   1. Introduction.
   2. Special aspects associated with lung microdialysis compared to microdialysis in other tissues.
   3. Insertion of microdialysis probes into lung tissue.
   4. Insertion of microdialysis probes in the bronchial system.
   5. Types of probes.
   6. Endogenous compounds.
   7. Exogenous drugs.
   8. Animal data.
   9. Clinical data.
   10. Comparison of pharmacokinetic data in lung obtained by microdialysis and other techniques.
   11. Predictability of lung concentrations by measurements in other tissues.
8. Microdialysis in the hepatobiliary system: Monitoring drug metabolism, hepatobiliary excretion and enterohepatic circulation.
   1. Introduction.
   2. Experimental considerations of pharmacokinetic studies.
   3. Pharmacokinetic and hepatobiliary excretion studies employing microdialysis.
   4. Conclusions.
9. Microdialysis on the metabolism of glucose, lactate and glycerol.
   1. Introduction.
   2. Glucose.
   3. Lactate.
   4. Lactate/pyruvate ratio.
   5. Glycerol.
10. Clinical microdialysis in skin and soft tissues.
    1. Introduction.
    2. Tissue Bioavailability.
    3. PK/PD Indices.
    4. Topical Bioequivalence.
    5. Endogenous Compounds.
    6. Conclusion.
11. Microdialysis on Adipose Tissue: Monitoring Tissue Metabolism and Blood Flow in Humans.
    1. Introduction.
    2. Principle and Practical Considerations for Application of Microdialysis on Adipose Tissue.
    3. Application of Microdialysis on Adipose Tissue in Humans.
    4. Summary and Conclusions.
12. Microdialysis as a monitoring system for human diabetes.
    1. Introduction.
    2. Monitoring of the acute complications of diabetes.
13. Microdialysis in Tumors: Drug Disposition and Tumor Response.
    1. Introduction.
    2. Considerations of microdialysis as a sampling technique in oncology.
    3. Experimental considerations.
    4. Examples of using microdialysis techniques to characterize drug disposition in tumor.
    5. Application of microdialysis in evaluation of tumor response to therapy.
    6. Conclusions and future perspective.
14. Microdialysis versus imaging techniques for in vivo drug distribution measurements.
    1. Introduction.
    2. Microdialysis.
    3. Imaging techniques.
    4. Magnetic resonance imaging and magnetic resonance spectroscopy.
    5. Positron Emission Tomography (PET).
    6. Combination of Microdialysis and Imaging Techniques.
    7. Summary and Conclusion.
15. In vitro applications of microdialysis.
    1. Introduction.
    2. Microdialysis applied in culture systems.
    3. Microdialysis applied in enzyme kinetics.
    4. Microdialysis applied in protein binding.
    5. Conclusions.
16. Microdialysis in Drug-Drug Interaction.
    1. Introduction.
    2. Pharmacokinetic drug interaction.
    3. Pharmacodynamic drug interaction.
    4. Conclusions.
17. Microdialysis in environmental monitoring.
    1. Introduction.
    2. In-vivo sampling vis-à-vis in-situ sampling: Similarities and differences.
    3. Critical parameters influencing relative recoveries.
    4. Detection techniques.
    5. Calibration methods.
    6. Environmental applications of microdialysis.
    7. Conclusions and future trends.

AUTHOR

Tung-Hu Tsai, PhD, is a Professor at the Institute of Traditional Medicine, National Yang-Ming University, Taiwan. He has published more than 200 papers in the field of pharmaceutical analysis, herbal medicine, and pharmacokinetics. Dr. Tsai's current research focuses on the mechanisms of pharmacokinetic pathways, emphasizing hepatobiliary excretion and barrier transportation.