Intermediate Filament Associated Proteins (Methods in Enzymology, Vol. 569)

Description

Intermediate Filament Associated Proteins, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in intermediate filament associated proteins and contains sections on such topics as lamin-associated proteins, intermediate filament-associated proteins and plakin, and other cytoskeletal cross-linkers.

Key Features

• Continues the legacy of this premier serial with quality chapters authored by leaders in the field
• Covers research methods in intermediate filament associated proteins and contains sections on such topics as lamin-associated proteins, intermediate filament-associated proteins and plakin, and other cytoskeletal cross-linkers

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.

Contents

Part I: Identification and Biochemical Analysis of Lamin-Associated Proteins

1. BioID Identification of Lamin-Associated Proteins
Aaron A. Mehus, Ruthellen H. Anderson and Kyle J. Roux

2. Proximity-Dependent Biotin Identification (BioID) in Dictyostelium Amoebae
Petros Batsios, Irene Meyer and Ralph Gräf

3. Purification and Structural Analysis of LEM-Domain Proteins
Isaline Herrada, Benjamin Bourgeois, Camille Samson, Brigitte Buendia, Howard J Worman and Sophie Zinn-Justin

4. Purification and Structural Analysis of SUN and KASH Domain Proteins
F. Esra Demircioglu, Victor E. Cruz and Thomas U. Schwartz

5. Purification of Lamins and Soluble Fragments of NETs
Alexandr A. Makarov, Andrea Rizzotto, Peter Meinke and Eric C. Schirmer

6. Simple Separation of Functionally-Distinct Populations of Lamin-Binding Proteins
Jason M. Berk and Katherine L. Wilson

Part II: General Methods for Analysis of Intermediate Filament-Associated Proteins

7. Development of a Novel Green Fluorescent Protein-Based Binding Assay to Study the Association of Plakins with Intermediate Filament Proteins
Bertrand Favre, Nadja Begré, Jamal-Eddine Bouameur, and Luca Borradori

8. Functional Analysis of Keratin-Associated Proteins in Intestinal Epithelia: Heat- Shock Protein Chaperoning and Kinase Rescue
Anastasia Mashukova, Radia Forteza and Pedro J. Salas

9. Purification of Protein Chaperones and their Functional Assays with Intermediate Filaments
Ming Der Perng, Yu-Shan Huang and Roy A. Quinlan

10. Purification and Structural Analysis of Plectin and BPAG1e
José A Manso, Inés García Rubio, María Gómez-Hernández, Esther Ortega, Rubén M Buey, Ana M Carballido, Arturo Carabias, Noelia Alonso-García and José M de Pereda

11. Purification and Structural Analysis of Desmoplakin
Hee-Jung Choi and William I. Weis

12. Degradation of the Intermediate Filament Family by Gigaxonin
Pascale Bomont

Part III: Functional and Genetic Analysis of the Plakin and Other Cytoskeletal Cross-Linkers

13. Functional and Genetic Analysis of Plectin in Skin And Muscle
Günther A. Rezniczek, Lilli Winter, Gernot Walko, and Gerhard Wiche

14. Functional and Genetic Analysis of Epiplakin in Epithelial Cells
Sandra Szabo, Karl L. Wögenstein and Peter Fuchs

15. In Vitro Model of the Epidermis: Connecting Protein Function to 3D Structure
Christopher Arnette,Jennifer L. Koetsier, Paul Hoover, Spiro Getsios and Kathleen J. Green

16. Functional Analysis of Periplakin and Envoplakin, Cytoskeletal Linkers and Cornified Envelope Precursor Proteins
Veronika Boczonadi and Arto Määttä

17. Microtubule-Actin Crosslinking Factor 1: Domains, Interaction Partners and Tissue-Specific Functions
Dmitry Goryunov and Ronald K. H. Liem

18. Functional and Genetic Analysis of Neuronal Isoforms of BPAG1
Anisha Lynch-Godrei and Rashmi Kothary

19. "Functional and Genetic Analysis of Spectraplakins in Drosophila”
Ines Hahn, Matthew Ronshaugen, Natalia Sánchez-Soriano and Andreas Prokop

20. Functional and Genetic Analysis of VAB-10 Spectraplakin in C. Elegans
Christelle Gally, Huimin Zhang and Michel Labouesse

Part IV: Functional and Genetic Analysis of Lamin-associated proteins

21. Tagged Chromosomal Insertion Site (TCIS) System, a Method to Study Lamina Associated Chromatin
Jennifer C. Harr and Karen L. Reddy

22. Lamin Binding Proteins in Caenorhabditis Elegans
Agnieszka Dobrzynska, Peter Askjaer and Yosef Gruenbaum

23. Pathologically-Relevant Prelamin A Interactions with Transcription Factors
Arantza Infante and Clara I. Rodríguez

24. MCLIP Detection of Novel Protein-Protein Interactions at the Nuclear Envelope
Mohammed Hakim Jafferali, Ricardo A. Figueroa and Einar Hallberg

25. Preparation of the Human Cytomegalovirus Nuclear Egress Complex and Associated Proteins
Mayuri Sharma, Jeremy P. Kamil and Donald M. Coen

Authors

Katherine Wilson
Department of Cell Biology, The Johns Hopkins University School of Medicine, USA

Arnoud Sonnenberg
Department of Cell Biology, The Netherlands Cancer Institute, The Netherlands