Quantitative Imaging in Cell Biology (Methods in Cell Biology, Vol. 123)

Features:

• Covers sections on model systems and functional studies, imaging-based approaches and emerging studies
• Chapters are written by experts in the field
• Cutting-edge material

Table of Contents:

1. Concepts in Quantitative Fluorescence Microscopy
Jennifer C. Waters and Torsten Wittmann
2. Practical Considerations of Objective Lenses for Application in Cell Biology
Stephen T. Ross, John R. Allen and Michael W. Davidson
3. Assessing Camera Performance for Quantitative Microscopy
Talley J. Lambert and Jennifer C. Waters
4. A Practical Guide to Microscope Care and Maintenance
Lara J. Petrak and Jennifer C. Waters
5. Fluorescence Live Cell Imaging
Andreas Ettinger and Torsten Wittmann
6. Fluorescent Proteins for Quantitative Microscopy: Important Properties and Practical Evaluation
Nathan Christopher Shaner
7. Quantitative Confocal Microscopy: Beyond a Pretty Picture
James Jonkman, Claire M. Brown and Richard W. Cole
8. Assessing and Benchmarking Multiphoton Microscopes for Biologists
Kaitlin Corbin, Henry Pinkard, Sebastian Peck, Peter Beemiller and Matthew F. Krummel
9. Spinning Disk Confocal Microscopy: Present Technology and Future Trends
John Oreopoulos, Richard Berman and Mark Browne
10. Quantitative Deconvolution Microscopy
Paul C. Goodwin
11. Light Sheet Microscopy
Michael Weber, Michaela Mickoleit and Jan Huisken
12. DNA Curtains: Novel Tools for Imaging Protein-nucleic Acid Interactions at the Single-molecule Level
Bridget E. Collins, Ling F. Ye, Daniel Duzdevich and Eric C. Greene
13. Nanoscale Cellular Imaging with Scanning Angle Interference Microscopy
Christopher DuFort and Matthew Paszek
14. Localization Microscopy in Yeast
Markus Mund, Charlotte Kaplan and Jonas Ries
15. Imaging Cellular Ultrastructure by PALM, iPALM, and Correlative iPALM-EM
Gleb Shtengel, Yilin Wang, Zhen Zhang, Wah Ing Goh, Harald F. Hess and Pakorn Kanchanawong
16. Seeing more with Structured Illumination Microscopy
Reto Fiolka
17. Structured Illumination Super-Resolution Imaging of the Cytoskeleton
Ulrike Engel
18. Analysis of Focal Adhesion Turnover: A Quantitative Live Cell Imaging Example
Samantha Stehbens and Torsten Wittmann
19. Determining Absolute Protein Numbers by Quantitative Fluorescence Microscopy
Jolien Suzanne Verdaasdonk, Josh Lawrimore, and Kerry Bloom
20. High Resolution Traction Force Microscopy
Sergey V. Plotnikov, Benedikt Sabass, Ulrich S. Schwarz and Clare M. Waterman
21. Experimenters’ Guide to Co-Localization Studies: Finding a Way Through Indicators and Quantifiers, in Practice
Fabrice P. Cordelières and Susanne Bolte
22. User-Friendly Tools for Quantifying the Dynamics of Cellular Morphology and Intracellular Protein Clusters
Denis Tsygankov, Pei-Hsuan Chu, Hsin Chen, Timothy C. Elston and Klaus Hahn
23. Ratiometric Imaging of pH Probes
Bree K. Grillo-Hill, Bradley A. Webb and Diane L. Barber
24. Towards Quantitative Fluorescence Microscopy with DNA Origami Nanorulers
Susanne Beater, Mario Raab and Philip Tinnefeld
25. Imaging and Physically Probing Kinetochores in Live Dividing Cells
Jonathan Kuhn and Sophie Dumont
26. Adaptive Fluorescence Microscopy by Online Feedback Image Analysis
Christian Tischer, Volker Hilsenstein, Kirsten Hanson and Rainer Pepperkok
27. Open Source Solutions for SPIMage Processing
Christopher Schmied, Evangelia Stamataki and Pavel Tomancak
28. Second Harmonic Generation Imaging of Cancer
Adib Keikhosravi, Jeremy S. Bredfeldt, Md Abdul Kader Sagar and Kevin W. Eliceiri

Jennifer Waters, Department of Cell Biology, Harvard Medical School, MA, USA and Torsten Wittmann, Department of Cell and Tissue Biology, University of California - San Francisco, USA