Laboratory Methods in Cell Biology: Imaging (Methods in Cell Biology, Vol. 113)

Description

This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as bioluminescent imaging of gene expressions, confocal imaging, and electron microscopy of bone.

Table of Contents

1. Single Molecule FRET Microscopy
   Victoria Birkedal (nee Ciulin)
2. Bioluminescent Imaging of Gene Expressions
   X. Chen
3. Confocal Imaging
   Anda Cornea
4. Electron Microscopy of Bone
   Vincent Everts
5. Immunostaining: Detection of Signaling Protein Location in Tissues, Cells and Subcellular Compartments
   Rory Fisher
6. Bimolecular Fluorescence Complementation (BiFC) Analysis of Protein-Protein Interactions
   Changdeng Hu and Yutaka Kodama
7. Histochemistry
   Lothar Jennes
8. Live Imaging
   Naoto Kawakami
9. Click Labeling for Single-Molecule FRET
   E.A. Lemke
10. High Throughput Methods for the Detection of Protein Over Expression Using Fluorescent Markers
    Karen McLuskey & Mads Gabrielsen
11. Correlation Microscopy
    Sasha Mironov
12. Stereology of the Lung
    Matthias Ochs & Jan Philipp Schneider
13. Formation and Preparation of Cellular Spheroids for High-Content Assays Based on Epifluorescence and Laser Light Sheet Fluorescent Microscopies.
    Francesco Pampaloni, Nariman Ansari & Ernst Stelzer
14. Optimising Fluorochrome Excitation and Detection for Intravital 2-Photon Microscopy
    Tri Giang Phan
15. Cryomicroscopy
    Ingeborg Schmidt-Krey
16. In situ Hybridization
    Chung-Jui Tsai
17. Imaging RNA
    Luis Vaca Dominguez

Author Info

P. Michael Conn, Oregon Regional Primate Research Center, Beaverton, USA