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Cell Biology Assays. Proteins
Celis, Julio
1ª Edición Noviembre 2009
Inglés
394 pags
1100 gr
null x null x null cm
ISBN 9780123756923
Editorial ACADEMIC PRESS
Contents
Protein Determination
Phosphopeptide Mapping: A Basic Protocol
Coupling of Fluorescent Tags to Proteins
Radioiodination of Proteins and Peptides
Free-Flow Electrophoresis
Gel-Based Proteomics: High-Resolution Two-Dimensional Gel Electrophoresis of
Proteins. Isoelectric Focusing and Nonequalibrium pH Gradient Electrophoresis.
High-Resolution Two-Dimensional Electrophoresis with Immobilized pH Gradients
for Proteome Analysis
Two-Dimensional Difference Gel Electrophoresis: Application for the Analysis
of Differential Protein Expression in Multiple Biological Samples
Affinity Electrophoresis for Studies of Biospecific Interations: High-Resolution
Two-Dimensional Affinity Electrophoresis for Separation of Hapten-Specific Polyclonal
Antibodies into Monoclonal Antibodies in Murine Blood Plasma
Image Analysis and Quantitation
Fluorescence Detection of Proteins in Gels Using SYPRO Dyes
Autoradiography and Fluorography: Film-Based Techniques for Imaging Radioactivity
in Flat Samples
Two-Dimensional Gel Profiling of Posttanslationally Modified Proteins by in
vivo Isotope Labeling
Immunoprecipitation of Proteins under Nondenaturing Conditions
Nondenaturing Polyacrylamide Gel Electrophoresis as a Method for Studying Protein
Interations: Applications in the Analysis of Mitochrondrial Oxidative Phosphorylation
Complexes
Affinity purification with Natural Immobilized Ligands
Analysis of Protein-Protein Interactions by Chemical Cross-Linking
Peroxisomal Targeting as a Tool to Assess Protein-Protein Interactions
Biomolecular Interaction Analysis Mass Spectrometry
Blot Overlays with P-Labedled GST-Ras Fusion Proteins: Application to Mapping
Protein-Protein Interaction Sites
Ligand Blot Overlay Assay: Detetion of Ca+2- and Small GTP-Binding Proteins
Modular Scale Yeast Two-Hybrid Screening
Chromophore-Assisted Laser Inactivation of Proteins by Antibodies Labeled with
Malachite Green
Chromatin Immunoprecipitation (ChIP)
Gel Mobility Shift Assay
DNA Affinity Chromatography of Transcription Factors: The Oligaonucleotide Trapping
Approach
Protein Degradation Methods: Chaperone-Mediated Autophagy
Methods in Protein Ubiquitination
Protein Identification and Sequencing by Mass Spectrometry
Proteome Specific Sample Preparation Methods for Matrix-Assisted Laser Desorption/Ionization
Mass Spectrometry
In-Gel Digestion of Protein Spots for Mass Spectrometry
Peptide Sequencing by Tandem Mass Spectrometry
Direct Database Searing Using Tandem Mass Spectra of Peptides
Identification of Proteins from Organisms with Unsequenced Genomes by Tandem
Mass Spectrometry and Sequence-Similarity Database Searching Tools
Identification of Protein Phosphorylation Sites by Mass Spectrometry
Analysis of Carbohydrates/Glycoproteins by Mass Spectrometry
Stable Isotope Labeling by Amindo Acids in Cell Culture for Quantitative Proteomics
Site-Specific, Stable Isotopic Labeling of Cysteinyl Pepties in Complex Peptide
Mixtures
Protein Hydrogen Exchange measured by Electrospry Ionization Mass Spectrometry
Nongel Based Proteomics: Selective Reversed-Phase Chromatographic Isolation
of Methionine-Containing Peptides from Complex Peptide Mixtures
Mass Spectrometry in Noncovalent Protein Interactions and Protein Assemblies
Readership
Graduate students and professionals in a lab enviornment
Author Information
Julio E. Celis, Danish Cancer Society; Institute of Cancer Biology and Danish
Centre for Human Genome Research; Copenhagen, Denmark
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