Structural Biology Using Electrons and X-Rays. an Introduction for Biologists

Edited by Michael F Moody

Description:

Structural Biology Using Electrons and X-rays is the perfect book to provide advanced undergraduates or graduate students with an accessible introduction to the two major diffraction-based techniques of structural biology. While concentrating on electron cryo-microscopy with image analysis, it also includes X-ray crystallography in a coherent survey of fundamental principles. Starting with Fourier transforms and progressing through optics and imaging principles, symmetry and three-dimensional reconstruction theory, Structural Biology Using Electrons and X-rays ends in an account of the latest methods adapted to crystals, helices, viral heads, and single particles.

Features:

• Emphasis on common principles underlying all diffraction-based methods
• Thorough grounding in theory requires understanding of only simple algebra
• Visual representations and explanations of challenging content
• Mathematical detail offered in short-course form to parallel the text

Table of Contents:

Part I: Fourier Transforms

1. Overview
2. Correlations & Convolutions
3. Fourier Fundamentals
4. Digital Fourier Transforms
5. Filters
6. Two-Dimensional FTs

Part II: Optics

7. Microscopy with Rays
8. Waves
9. Wave Imaging

Part III: Further Mathematical Methods/General Aspects

10. Symmetry
11. Statistics & Matrices
12. The Third Dimension

Part IV: Image Analysis/Structure Determination

13. Special Symmetries
14. Crystalline Sheets
15. Helices
16. Icosahedral Particles
17. Unsymmetrical ("Single") Particles
18. Distortion Correction
19. FT Mathematics
20. Elementary Matrices