Gene Cloning and DNA Analysis. An Introduction

Tabla de contenidos

• Preface to the Ninth Edition
• About the Companion Website
• Part I. The Basic Principles of Gene Cloning and DNA Analysis
  • 1. Why Gene Cloning and DNA Analysis Are Important?
  • 2. Vectors for Gene Cloning: Plasmids and Bacteriophages
  • 3. Purification of DNA from Living Cells
  • 4. Manipulation of Purified DNA
  • 5. Introduction of DNA into Living Cells
  • 6. Cloning Vectors for Escherichia coli
  • 7. Cloning Vectors for Eukaryotes
  • 8. How to Obtain a Clone of a Specific Gene
  • 9. The Polymerase Chain Reaction
• Part II. The Applications of Gene Cloning and DNA Analysis in Research
  • 10. Sequencing Genes and Genomes
  • 11. Studying Gene Expression and Function
  • 12. Studying Genomes
  • 13. Studying Transcriptomes and Proteomes
  • 14. DNA Editing
• Part III. The Applications of Gene Cloning and DNA Analysis in Biotechnology
  • 15. Production of Protein from Cloned Genes
  • 16. Gene Cloning and DNA Analysis in Medicine
  • 17. Gene Cloning and DNA Analysis in Agriculture
  • 18. Gene Cloning and DNA Analysis in Forensic Science and Archaeology
• Glossary
• Index

Descripción

Gene Cloning and DNA Analysis, Ninth Edition is a world-renowned introductory text offering a comprehensive exploration of the rapidly growing and dynamic field of gene cloning and DNA analysis. Presented in full color with an easy-to-follow layout and over 250 clear illustrations, this edition provides accessible learning for a wide audience in the biological sciences.

This newly revised and updated edition highlights advances in DNA sequencing technologies and expands coverage on DNA editing, including CRISPR methodology and its applications in gene therapy and plant genetic engineering. Additional updates include optical mapping of DNA molecules, in situ transcriptome analysis, RNA interference techniques for crop plants, studying kinship by DNA profiling, and the use of ancient DNA to investigate human prehistory and palaeogenomics.

Gene Cloning and DNA Analysis also covers topics such as:

• Strategies for assembling telomere-to-telomere chromosome sequences
• Synthesis of recombinant vaccines against diseases such as COVID-19 and hepatitis B
• Interbreeding between Neanderthals and Homo sapiens
• Ethical issues surrounding gene cloning and DNA editing in pharming, gene therapy, and plant genetic modification

The Ninth Edition remains an essential reference for undergraduate and graduate students in genetics and genomics, molecular biology, biochemistry, immunology, and applied biology, as well as for professionals across all areas of biological science.