Molecular Aspects of Hematologic Malignancies. Diagnostic Tools and Clinical Applications (Principles and Practice)

ABOUT THIS BOOK

• Up-to-date information on the molecular basis of selected hematologic disorders
• Practical reference for molecular laboratory procedures
• Of interest to clinical oncologists and hematologists as well as to graduates in molecular medicine, human genetics and laboratory medicine

This book provides a state-of-the-art approach to the molecular basis of hematologic diseases and its translation into improved diagnostics and novel therapeutic strategies. Several representative hemato-oncologic malignancies are analyzed in detail: acute lymphoblastic leukemia, acute myeloid leukemia, B-cell Non-Hodgkin lymphomas, multiple myeloma, chronic lymphocytic leukemia, chronic myeloid leukemia, myelodysplastic syndromes, and myeloproliferative neoplasms. Experts in the field describe the molecular methods applied for modern diagnostics and therapies, such as hematopoietic stem cell transplantation, donor recipient matching, banking of biological material, analyses of post-transplant chimerism, and minimal residual disease monitoring. The volume concludes with an extensive section comprising thorough step-by-step protocols of molecular techniques in hematology, all of them validated in the authors’ own laboratories.

Content Level » Research
Keywords » hematology - leukemia - minimal residual disease - molecular biology - oncology - stem cell transplantation
Related subjects » Biomedical Sciences - Cancer Research - Human Genetics - Internal Medicine - Molecular Medicine

TABLE OF CONTENTS

Part I MOLECULAR BIOLOGY OF SELECTED HEMATO-ONCOLOGIC DISEASES

• 1. Molecular biology of acute lymphoblastic leukemia
  Małgorzata Dawidowska, Monika D. Kraszewska, Katarzyna Derwich, and Tomasz Szczepański
• 2. Genetic mechanisms and molecular markers of neoplastic transformation in acute myeloid leukemia
  Agata A. Filip, Marta Libura, Sebastian Giebel, and Olga Haus
• 3. Molecular pathogenesis of aggressive B-cell lymphomas
  Przemysław Juszczyński and Krzysztof Warzocha
• 4. Molecular biology and classification of multiple myeloma
  Anna Dmoszyńska and Norbert Grząśko
• 5. Chronic lymphocytic leukemia
  Anton W. Langerak and Yorick Sandberg
• 6. The molecular biology of chronic myeloid leukemia
  Tomasz Sacha, Kajetana Foryciarz, and Aleksander B. Skotnicki
• 7. Molecular biology of myelodysplastic syndromes
  Francesc Solé
• 8. Myeloproliferative neoplasms
  Andrzej Hellmann, Maria Bieniaszewska, Witold Prejzner, and Aleksandra Leszczyńska

Part II DIAGNOSTICS AND MONITORING OF THERAPY OF HEMATO-ONCOLOGIC DISEASES

• 9. Flow cytometric immunophenotyping as diagnostic tool of hematopoietic malignancies
  Łukasz Sędek, Juan Flores-Montero, Joanna Bulsa, Susana Barrena, Julia Almeida, Alberto Orfao, and Tomasz Szczepański
• 10. Cytogenetics in hematology
  Olga Haus, Anna Poluha, and Katarzyna Skonieczka
• 11. Monitoring of minimal residual disease in acute lymphoblastic leukemia
  Tomasz Szczepański, Małgorzata Dawidowska, and Katarzyna Derwich
• 12. Gene expression profiling in hematologic malignancies
  Przemysław Juszczyński, Bjoern Chapuy, Małgorzata Szczepaniak, and Krzysztof Warzocha

Part III MOLECULAR PROCEDURES INVOLVED IN HEMATOPOIETIC STEM CELL TRANSPLANTATION

• 13. Allogeneic transplantation of hematopoietic stem cells
  Jacek Wachowiak
• 14. Genetic basis of donor-recipient matching in allogeneic transplantation of hematopoietic stem cells
  Jacek Nowak and Jacek Wachowiak
• 15. Chimerism following allogeneic transplantation of hematopoietic stem cells
  Małgorzata Dawidowska, Katarzyna Guz, Ewa Brojer, Jacek Wachowiak, and Michał Witt
• 16. Peritransplantation monitoring of minimal residual disease in acute lymphoblastic leukemia
  Małgorzata Dawidowska, Katarzyna Derwich, and Tomasz Szczepański
• 17. Biobanks of cellular material
  Wiesław Wiktor-Jedrzejczak and Elzbieta Urbanowska

Part IV MOLECULAR DIAGNOSTIC PROTOCOLS

• 18. Biobanking of cellular material
  Anna Poluha and Elżbieta Urbanowska
• 19. Isolation of mononuclear cells from human blood and bone marrow by density gradient centrifugation
  Małgorzata Dawidowska
• 20. Molecular techniques commonly used in hematooncolology
  Monika D. Kraszewska and Ewa Ziętkiewicz
• 21. Genetic methods of HLA typing
  Jacek Nowak, Renata Mika-Witkowska, and Elżbieta Graczyk-Pol
• 22. Post-transplant chimerism analysis through STR-PCR and RQ-PCR
  Małgorzata Dawidowska, Katarzyna Guz, Agnieszka Orzińska, Justyna Smolarczyk-Wodzyńska, Monika D. Kraszewska, and Michał Witt
• 23. Analysis of minimal residual disease with the use of rearrangements of Ig/TCR genes through RQ-PCR
  M. Dawidowska, Vincent H. J. van der Velden, Michał Witt, and Tomasz Szczepański
• 24. Molecular diagnostics of acute myeloblastic leukemia
  Marta Libura, Agata A. Filip, and Olga Haus
• 25. Assessment of the presence and the level of BCR-ABL fusion gene expression and mutational status in ABL kinase domain.
  Sylwia Czekalska, Magdalena Zawada, and Izabela Florek
• 26. Studies of rearrangements and somatic hypermutation of IGHV genes in chronic lymphocytic leukemia
  Anton W. Langerak, Richard Rosenquist, Paolo Ghia, Chrysoula Belessi, Kostas Stamatopoulos, and Frederic Davi for the ERIC-IgCLL group
• 27. Molecular biology methods in the diagnosis of multiple myeloma
  Anna Dmoszyńska and Sylwia Chocholska
• 28. Molecular methods in myeloproliferative neoplasms
  Aleksandra Leszczyńska, Witold Prejzner, Maria Bieniaszewska, and Andrzej Hellmann
• 29. Informed consent for participation in research project
  Małgorzata Dawidowska and Michał Witt