Cell-Free Protein Synthesis. Methods and Protocols

Description

With its detailed description of membrane protein expression, high-throughput and genomic-scale expression studies, both on the analytical and the preparative scale, this book covers the latestadvances in the field. The step-by-step protocols and practical examples given for each method constitute practical advice for beginners and experts alike. 

Table of contents

1. Large scale batch reactions for cell-free protein synthesis (J. R. Swartz, A. Spirin)
2. The constructive approach for cell-free translation (T. Ueda)
3. Functional genomic analysis using sequential cell-free protein synthesis (J. R. Swartz)
4. Cell-free technology for rapid production of patient-specific fusion protein vaccines (A. R. Goerke, J. Yang, G. Kanter, R. Levy and J. R. Swartz)
5. Bacterial cell-free system for highly efficient protein synthesis (T. Kigawa, T. Matsuda, T. Yabuki, S. Yokoyama)
6. The use of the Escherichia-coli cell-free protein synthesis for structural biology and structural proteomics (T. Kigawa, M. Inoue, M. Aoki, T. Matsuda, T. Yabuki, E. Seki, T. Harada, S.Watanabe, S. Yokoyama)
7. The wheat germ cell-free protein synthesis system (T. Sawasaki, Y. Endo)
8. Cell-free expression of integral membrane proteins for structural studies (C. Klammt, D. Schwarz, I. Lehner, S. Sobhanifar, F. Löhr, J. Zeelen, C. Glaubitz, V. Dötsch, F. Bernhard)
9. Cell-free production of membrane protein in presence of detergents (J. Betton, M. Miot)
10. Novel techniques using PCR and cell-free protein synthesis systems for combinatorial bioengineering (H. Nakano, T. Yamane)
11. Gene Cloning and Expression in Molecular Colonies (A. B. Chetverin, T. R. Samatov, H. V. Chetverina)