


Cell-Free Protein Synthesis. Methods and Protocols
Spirin, S. — Swartz, J.
1ª Edición Octubre 2007
Inglés
Tapa dura
262 pags
612 gr
18 x 25 x 2 cm
ISBN 9783527316496
Editorial WILEY
Recíbelo en un plazo De 7 a 10 días
Description
With its detailed description of membrane protein expression, high-throughput and genomic-scale expression studies, both on the analytical and the preparative scale, this book covers the latestadvances in the field. The step-by-step protocols and practical examples given for each method constitute practical advice for beginners and experts alike.
Table of contents
- Large scale batch reactions for cell-free protein synthesis (J. R. Swartz, A. Spirin)
- The constructive approach for cell-free translation (T. Ueda)
- Functional genomic analysis using sequential cell-free protein synthesis (J. R. Swartz)
- Cell-free technology for rapid production of patient-specific fusion protein vaccines (A. R. Goerke, J. Yang, G. Kanter, R. Levy and J. R. Swartz)
- Bacterial cell-free system for highly efficient protein synthesis (T. Kigawa, T. Matsuda, T. Yabuki, S. Yokoyama)
- The use of the Escherichia-coli cell-free protein synthesis for structural biology and structural proteomics (T. Kigawa, M. Inoue, M. Aoki, T. Matsuda, T. Yabuki, E. Seki, T. Harada, S.Watanabe, S. Yokoyama)
- The wheat germ cell-free protein synthesis system (T. Sawasaki, Y. Endo)
- Cell-free expression of integral membrane proteins for structural studies (C. Klammt, D. Schwarz, I. Lehner, S. Sobhanifar, F. Löhr, J. Zeelen, C. Glaubitz, V. Dötsch, F. Bernhard)
- Cell-free production of membrane protein in presence of detergents (J. Betton, M. Miot)
- Novel techniques using PCR and cell-free protein synthesis systems for combinatorial bioengineering (H. Nakano, T. Yamane)
- Gene Cloning and Expression in Molecular Colonies (A. B. Chetverin, T. R. Samatov, H. V. Chetverina)
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