Intended for readers with a background in fertility medicine as well as those less familiar with IVF, this comprehensive work presents an update on preimplantation genetic testing to enable single embryo transfer (SET). An international cast of contributors explains the treatment sequence—from ovulation induction to luteal support—aiming to transfer only one euploid embryo. Applications of molecular techniques for gamete and embryo assessment are fully detailed, with a focus on the strengths and limitations of each. In addition, expert commentary is shared across a range of regulatory challenges associated with embryo screening and cryopreservation. As access to advanced reproductive technology increases against a sharper background of healthcare reform, clinicians, economists, bioethicists and legislators alike will find this new volume relevant and highly accessible.
- Provides explanation of each step of the process -- from ovulation induction to embryo transfer
- Maximizes reader insights into the value of transferring a single embryo and explores ethical and regulatory issues in single embryo IVF transfer
- Details and assesses the molecular techniques for embryo evaluation
The Development of PGD.- Elements of Informed Consent for Preimplantation Genetic Diagnosis.- Follicular Recruitment & Oocyte Recovery in Assisted Reproduction.- Biomarker-based Flow-Cytometric Semen Analysis for Male Infertility Diagnostics and Clinical Decision Making in ART.- Comparison of Methods for Assessment of Sperm DNA Damage (Fragmentation) and Implications for the Assisted Reproductive Technologies.- Tweaking ICSI for a PGD program.- Comprehensive Chromosomal Screening from Polar Body Biopsy to Blastocyst Trophectoderm Sampling: Evidence and Considerations.- Polar Body Diagnosis—An Alternative and Addition to Preimplantation Diagnosis.- Efficiency of Polar Body Biopsy for Aneuploidy Screening by DNA Microarray for Single Embryo Transfer.- Prediction of Embryo Viability by Morphokinetic Evaluation to Facilitate Single Transfer.- Comparison of PCR Techniques for Embryo Screening and Single Embryo Transfer.- Array CGH and Partial Genome Sequencing for Rapidly Karyotyping IVF Blastocysts Before Transfer.- Single Embryo Transfers Enabled by aCGH—Current and Future Methodologies.- SNP array, qPCR, and next-generation sequencing based comprehensive chromosome screening.- Expanding PGD Applications to Non-traditional Genetic and Non-Genetic Conditions.- Should molecular cytogenetic techniques be applied to facilitate single embryo transfer in egg donation cases? Assessment of frequency and distribution of embryo aneuploidy after anonymous donor oocyte IVF.- Selecting the Single Embryo for Transfer in Mitochondrial Disorders.- Single Embryo Transfer: The Significance of the Embryo Transfer Technique.- The Vitrification Component: An Integral Part of a Successful eSET Program.- A Review of Luteal Support Protocols for Single Embryo Transfers--Fresh and Frozen.- Cost effectiveness of single embryo transfers relative to higher embryo transfer policies in clinical practice: A population based analysis.- The Quebec experience—One plus one equals two at once: Presenting cumulative pregnancy rates as the ideal outcome in elective SET programs.- Single Embryo Transfer in a State-Sponsored IVF Context: Current Experience from Israel.- Single Embryo Transfer: the Quebec Experience.- Regulatory Aspects of Embryo Testing: An American View.- Regulatory Challenges in Assisted Reproduction: A European Perspective.- Preimplantation Genetic Screening: Ethics & Policy.- Crossing Rubicons: Assisted Reproductive Therapies and Remaining Human.
Eric Scott Sills received his undergraduate degree from Vanderbilt and holds a PhD (molecular biology) from London’s University of Westminster. Dr. Sills was awarded the MD degree from University of Tennessee in 1992. After a gynecology residency at NYU Downtown Hospital, he completed his sub-specialty training in reproductive endocrinology at Cornell University. Having authored more than 100 peer-reviewed publications, Dr. Sills served as editor-in-chief for the Journal of Experimental & Clinical Assisted Reproduction from 2004–2014. He is board-certified by the American Board of Obstetrics and Gynecology, and is a Fellow of the American College of Obstetricians and Gynecologists, American College of Surgeons, Royal College of Physicians (Ireland), and Society of Biology (UK). At present, Dr. Sills is Medical Director at the Center for Advanced Genetics, an in vitro fertilization program based in Carlsbad, California. He is registered and/or licensed for medicine in California, New York and the United Kingdom.