


Auditory and Vestibular Research. Methods and Protocols (Methods in Molecular Biology 493)
Sokolowski, B.
ISBN-13: 9781934115626
HUMANA PRESS
Enero / 2009
1ª Edición
Inglés
Tapa dura
428 pags
1600 gr
20 x 27 x 2 cm
Recíbelo en un plazo De 2 a 3 semanas
About this book
While early studies of the auditory/vestibular sciences provided insights into the anatomy and neurophysiology of these systems and produced a prosthetic cochlear implant, the rise of molecular biology now permits a clear examination of the genetic basis for various hearing and balance disorders. In Auditory and Vestibular Research: Methods and Protocols, specialists in the field describe current RNA, protein, and imaging protocols that have provided insights into genetic regulation as well as a greater understanding of the genes and pathogens involved in diseases of the ear. This overview utilizes both mammalian and non-mammalian animal models as well as techniques applicable to clinical studies in order to best provide an up-to-date perspective on basic research. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Fluorescence Microscopy Methods in the Study of Protein Structure and Function
Heather Jensen-Smith, Benjamin Currall, Danielle Rossino, LeAnn Tiede, Michael Nichols, and Richard Hallworth
Ion Imaging in Cochlear Hair Cells
Gregory I. Frolenkov
Atomic Force Microscopy in Studies of the Cochlea
Michio Murakoshi and Hiroshi Wada
While early studies of the auditory/vestibular sciences provided insights into the anatomy and neurophysiology of these systems and produced a prosthetic cochlear implant, the rise of molecular biology now permits a clear examination of the genetic basis for various hearing and balance disorders. In Auditory and Vestibular Research: Methods and Protocols, specialists in the field describe current RNA, protein, and imaging protocols that have provided insights into genetic regulation as well as a greater understanding of the genes and pathogens involved in diseases of the ear. This overview utilizes both mammalian and non-mammalian animal models as well as techniques applicable to clinical studies in order to best provide an up-to-date perspective on basic research. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Auditory and Vestibular Research: Methods and Protocols is an ideal guide to the field and a useful tool for exploring genes and proteins in other systems as well, especially systems in which tissues are scarce and a comparative approach lends itself to discovering the underlying causes of human disorders.
Written for: Otolaryngologists, molecular biologists, pathologists
Keywords:
- Amino acid
- Balance disorders
- Hearing disorders
- Nucleic acid
- RT-PCR
Table of contents
Part 1: Nucleic Acid Protocols
- RNA Isolation from Xenopus Inner Ear Sensory Endorgans for Transcriptional
Profiling and Molecular Cloning
Casilda Trujillo-Provencio, TuShun R. Powers, David R. Sultemeier, and Elba E. Serrano - Synthesis of Biotin-Labeled RNA for Gene Expression Measurements Using Oligonucleotide
Arrays
Ana E. Vázquez, Liping Nie, and Ebenezer N. Yamoah - In situ Hybridization Approach to Study mRNA Expression and Distribution
in Cochlear Frozen Sections
Hakim Hiel - Lineage Analysis of Inner Ear Cells Using Genomic Tags for Clonal Identification
Takunori Satoh and Donna M. Fekete - Genetic Fate Mapping Approaches: New Means to Explore the Embryonic Origins
of the Cochlear Nucleus
Jun Chul Kim and Susan M. Dymecki - The Practical Use of Cre and loxP Technologies in Mouse Auditory Research
Yiling Yu and Jian Zuo - Helios® Gene Gun-Mediated Transfection of the Inner Ear Sensory Epithelium
Inna A. Belyantseva - Electroporation-Mediated Gene Transfer to the Developing Mouse Inner Ear
John V. Brigande, Samuel P. Gubbels, David W. Woessner, Jonathan J. Jungwirth, and Catherine S. Bresee - Isolation of Sphere-Forming Stem Cells from the Mouse Inner Ear
Kazuo Oshima, Pascal Senn, and Stefan Heller - Molecular Biology of Vestibular Schwannomas
Long-Sheng Chang and D. Bradley Welling - Multilocus Sequence Typing and Pulsed-Field Gel Electrophoresis of Otitis
Media Causing Pathogens
Jonathan C. Thomas and Melinda M. Pettigrew - Fluorescence "In situ" Hybridization for the Detection of Biofilm
in the Middle Ear and Upper Respiratory Tract Mucosa
Laura Nistico, Armin Gieseke, Paul Stoodley, Luanne Hall-Stoodley, Joseph E. Kerschner, and Garth D. Ehrlich - Positional Cloning of Deafness Genes
Hannie Kremer and Frans P. M. Cremers
Part II: Amino Acid Protocols
- Twist-off Purification of Hair Bundles
Jung-Bum Shin, James Pagana, and Peter G. Gillespie - Yeast Two-Hybrid Screening to Test for Protein-Protein Interactions in the
Auditory System
Dhasakumar S. Navaratnam - The Use of 2-D Gels to Identify Novel Protein-Protein Interactions in the
Cochlea
Thandavarayan Kathiresan, Margaret C. Harvey, and Bernd H. A. Sokolowski - Identification of Functionally Important Residues/Domains in Membrane Proteins
Using an Evolutionary Approach Coupled with Systematic Mutational Analysis
Lavanya Rajagopalan, Fred A. Pereira, Olivier Lichtarge, and William E. Brownell - In vivo Verification of Protein Interactions in the Inner Ear by Coimmunoprecipitation
Margaret C. Harvey and Bernd H. A. Sokolowski - Identification of Transcription Factor-DNA Interactions Using Chromatin
Immunoprecipitation Assays
Liping Nie, Ana E. Vazquez, and Ebenezer N. Yamoah - Surface Plasmon Resonance (SPR) Analysis of Binding Interactions of Proteins
in Inner-Ear Sensory Epithelia
Dennis G. Drescher, Neeliyath A. Ramakrishnan, and Marian J. Drescher - Multiplexed Isobaric Tagging Protocols for Quantitative Mass Spectrometry
Approaches to Auditory Research
Douglas E. Vetter, Johnvesly Basappa, and Sevin Turcan
Part III: Imaging Protocols
Heather Jensen-Smith, Benjamin Currall, Danielle Rossino, LeAnn Tiede, Michael Nichols, and Richard Hallworth
Gregory I. Frolenkov
Michio Murakoshi and Hiroshi Wada
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