Concepts and Techniques in Genomics and Proteomics

Summary

This book covers the important concepts of high-throughput modern techniques used in the genomics and proteomics field. Each technique is explained, with underlying concepts; simple line diagrams and flow charts are included to aid understanding and memory. A summary of key points precedes each chapter within the book, followed by detailed description in the subsections. Each subsection concludes with suggested relevant original references.

Key Features

• The book provides definitions for key concepts
• Case studies are included to illustrate ideas
• Important points to remember are noted
• The book contains suggestions for recent references and web site addresses
• Includes review questions and answers

Content

Part I: Genomics

• Introduction to genes and genomics
• Scope of Human Genome Project Genomes of model organisms: viral genomes; bacterial genomes; fungal genomes; plant genome; mouse genome; human genome
• Genome mapping: genetic mapping; cytogenetic mapping; physical mapping
• DNA sequencing methods: chemical degradation method; chain termination method of sequencing; advances in DNA sequencing Genome sequencing methods: hierarchical method of genome sequencing; shotgun method of genome sequencing; whole genome shot gun sequencing
• Genome sequence assembly techniques: clone contig preparation; pooling strategies; linking and jumping clones; closing gaps; draft and finished genome sequence
• Comparative genomics
• Functional genomics: Microarray; SAGE; DD-RT PCR; subtractive hybridization; RDA

Part II: Proteomics

• Introduction to proteomics
• Protein separation techniques
• Iso electric focusing of proteins
• SDS-PAGE separation of proteins
• Two dimensional gel electrophoresis of proteins
• Image acquisition and processing of 2D gels
• Edman sequencing of proteins
• Mass spectrometry for proteomics
• Pepetide mass finger printing
• Phosphoproteomics and glycoproteomics
• Systems biology

Readership

The book is primarily aimed towards undergraduate and postgraduate students within biotechnology, and would also be of interest to lecturers and research scholars. Academics in other fields, including life sciences, microbiology, biochemistry, medicine and bioinformatics, will also find the book of interest.

Author

Saraswathy, N. Dr and Ramalingam, P
Dr Saraswathy is currently Assistant Professor at the Department of Biotechnology, Kumaraguru College of Technology. She teaches courses on Molecular Biology, Genetic Engineering, and Plant Biotechnology, as well as Genomics and Proteomics. Dr Saraswathy gained her Ph.D. in Molecular Biology from the National Centre for Plant Biotechnology, New Delhi. She has published several articles in journals of biotechnology and biochemistry. Mr P Ramalingam is Assistant Professor at the Department of Biotechnology, Kumaraguru College of Technology. He received his M.Sc. in Biochemistry from Gulbarga University and MTech in Biotechnology from Anna University. He has also taught and researched in institutions such as Kongu Engineering College and PNC&KR College, and has contributed several published journal articles.