


Proteomics Methods Express
O'Connor, D.
1ª Edición Noviembre 2008
Inglés
Tapa blanda
256 pags
1000 gr
18 x 25 x 2 cm
ISBN 9781904842132
Editorial scion
Recíbelo en un plazo De 7 a 10 días
Proteomics: Methods Express identifies the most powerful new technologies and presents them in a way that allows their robust implementation.
The focus is on proteomic methods and strategies that are reliable and of general applicability. Each chapter presents descriptions of what can, and
cannot, be achieved with the relevant procedures so that readers can make informed judgments prior to establishing the methods in-house.
Every chapter discusses the merits and limitations of various approaches then provides tried-and-tested protocols with hints and tips for success and troubleshooting for when things go wrong.
Contents
1. Sample preparation and subcellular fractionation
approaches: purification of membranes and their microdomains for mass spectrometry
analysis
Yan Li , Phil Oh, and Jan E. Schnitzer, Sidney Kimmel Cancer Center
2. An isotope-coding strategy for quantitative proteomics
Xian Chen, Dept of Biochemistry and Biophysics, University of N. Carolina at
Chapel Hill
3. Gel-based approaches
Stuart Cordwell and Ben Crossett, both at School of Molecular and Microbial
Biosciences, University of Sydney, and Melanie Y. White, Minomic Pty Ltd
4. Peptide sorting by reverse-phase diagonal chromatography
Kris Gevaert and Jo 'ebl Vandekerckhove, Faculty of Medicine and Health Sciences,
Ghent University
5. Mass spectrometry strategies for protein identification
David R. Goodlett, University of Washington and Garry L. Corthals, University
of Turku
6. Desorption electrospray ionization: proteomics studies by a method that
bridges ESI and MALDI
Zoltan Takats, Justin M. Wiseman, Demian R. Ifa and R.Graham Cooks, all at Dept
of Chemistry, Purdue University
7. Analysis of cellular protein complexes by affinity purification and mass
spectrometry
Tilmann B 'fcrckst 'fcmmer and Keiryn L.. Bennett, both at Research Center for
Molecular Medicine of the Austrian Academy of Sciences
8. Clinical proteomic profiling and disease signatures
Rosamonde E. Banks, David A. Cairns, David N. Perkins and Jennifer H. Barrett,
all at Cancer Research UK Clinical Centre, St James' s University Hospital,
Leeds
9. Characterization of post-translational modifications: undertaking the phosphoproteome
W. Andy Tao, Purdue University; Bernd Bodenmiller and Ruedi Aebersold, both
Institute for Molecular Systems Biology, Federal Institute of Technology, Zurich
10. Protein microarray technologies
Chien-Sheng Chen, Sheng-Ce Tao, and Heng Zhu, Dept of Pharmacology and Molecular
Sciences, Johns Hopkins University School of Medicine
11. Intelligent mining of complex data: challenging the proteomic bottleneck
Dan Bach Kristensen, Maxygen and Alexandre Potelejnikov, Proxeon
12. Bioinformatic approaches in proteomics
Sandra Orchard and Henning Hermjakob, both European Bioinformatics Institute,
Hinxton
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