Human Cytomegaloviruses. Methods and Protocols (Methods in Molecular Biology, Vol. 1119)

ABOUT THIS BOOK

· Includes cutting-edge methods and protocols
· Provides step-by-step detail essential for reproducible results
· Contains key notes and implementation advice from the experts

With the significant medical role that human cytomegalovirus plays in human disease (from acute disease following primary infection, to chronic disease due to life-long viral persistence), the need to better understand human cytomegalovirus biology and pathogenesis is needed. Human Cytomegaloviruses: Methods and Protocols is designed to be an inclusive document covering all of the techniques and approaches necessary to understand and study the pathobiology of human cytomegalovirus. Topics covered include the history of human cytomegaloviruses, techniques to culture and grow the virus in model cell types, the use of primary cells for the study of human cytomegalovirus pathogenesis, modern molecular techniques for assessing the biological consequences of viral infection, animal models for study of cytomegalovirus replication and numerous other topics of current interest. Written in the successful Methods in Molecular Biologyseries format, each chapter includes an introduction, list of necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Human Cytomegaloviruses: Methods and Protocols serves as a tool for basic scientists as well as clinical scientists with an interest in the basic fundamental aspects of viral gene expression and specific aspects of viral pathogenesis.

Content Level » Professional/practitioner
Keywords » cytomegalovirus - fibroblasts - human cytomegaloviruses - pathobiology -pathogenesis
Related subjects » Internal Medicine - Virology

TABLE OF CONTENTS

1. History of the Molecular Biology of Cytomegalovirus
Mark F. Stinski

2. Overview of Human Cytomegalovirus Pathogenesis
Maciej T. Nogalski, Donna Collins-McMillen, and Andrew D. Yurochko

3. Distinct Properties of Human Cytomegalovirus Strains and the Appropriate Choice of Strains for Particular Studies
Giada Frascaroli and Christian Sinzger

4. Use of Diploid Human Fibroblasts as a Model System to Culture/Grow, Study and Understand Human Cytomegalovirus Infection
Elizabeth A. Fortunato

5. Use of Recombinant Approaches to Construct Human Cytomegalovirus Mutants
Iryna Dekhtiarenko, Luka Cicin-Šain and Martin Messerle

6. The Use of Primary Human Cells (Fibroblasts, Monocytes and Others) to Assess Human Cytomegalovirus Function
Emma Poole, Matthew Reeves, and John H. Sinclair

7. Human Hematopoietic Long-Term Culture (hLTC) for Human Cytomegalovirus Latency and Reactivation
Mahadevaiah Umashankar and Felicia Goodrum

8. Analysis of Cytomegalovirus Binding/Entry-Mediated Events
Gary C.T. Chan and Andrew D. Yurochko

9. Use of 5-Ethynyl-2’-Deoxyuridine Labelling and Flow Cytometry to Study Cell Cycle-Dependent Regulation of Human Cytomegalovirus Gene Expression
Lüder Wiebusch and Christian Hagemeier

10. Methods for Studying the Function of Cytomegalovirus GPCRs
Christine O’Connor and William E. Miller

11. Methods for the Detection of Cytomegalovirus in Glioblastoma Cells and Tissues
Charles S. Cobbs, Lisa Matlaf, and Lualhati E. Harkins

12. Methods to Study the Nucleocytoplasmic Transport of Macromolecules with Respect to Their Impact on the Regulation of Human Cytomegalovirus Gene Expression
Marco Thomas, Barbara Zielke, Nina Reuter, and Thomas Stamminger

13. Fluorescence-Based Laser Capture Microscopy Technology Facilitates Identification of Critical In Vivo Cytomegalovirus Transcriptional Programs
Craig N. Kreklywich, Patricia P. Smith, Carmen Baca Jones, Anda Cornea, Susan L. Orloff, and Daniel N. Streblow

14. Techniques for Characterizing Cytomegalovirus-Encoded miRNAs
Lauren M. Hook, Igor Landais, Meaghan H. Hancock, and Jay A. Nelson

15. What We Have Learned From Animal Models of HCMV
Pranay Dogra and Tim Sparer

16. Rodent Models of Congenital Cytomegalovirus Infection
Djurdjica Cekinovic, Vanda Juranic Lisnic, and Stipan Jonjic

17. Recent Approaches and Strategies in the Generation of Anti-Human Cytomegalovirus Vaccines
Suresh B. Boppana and William J. Britt

18. Approaches for the Generation of New Anti-Cytomegalovirus Agents: Identification of Protein-Protein Interaction Inhibitors and Compounds Against the HCMV IE2 Protein
Beatrice Mercorelli, Giorgio Gribaudo, Giorgio Palù, and Arianna Loregian