Haemostasis. Methods and Protocols (Methods in Molecular Biology, Vol. 992)

About this book

• Includes cutting-edge methods and protocols
• Provides step-by-step detail essential for reproducible results
• Contains key notes and implementation advice from the experts

The haemostatic system is one the most important physiological systems for maintaining health and well being, and thus the investigation of the haemostatic system remains a research priority. Disturbances of the haemostatic system in the broader sense, such as heart disease and strokes, arguably constitute the single greatest contribution to non-infectious mortality in the world today. Therefore, understanding the laboratory methods to assess the haemostatic system is vital for the practice of complex clinical medicine. In Haemostasis: Methods and Protocols, experts in the field address the major components of the haemostatic system, general principles of haemostatic testing, and techniques used to assess various aspects of the haemostatic system, grouped according to their functional indications. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Haemostasis: Methods and Protocols provides an ideal guide to scientists of all backgrounds and serves an urgent need for further research to develop superior methods of assessing the haemostatic system in humans.

Table of contents

Part I. The Haemostatic System

• 1. The Coagulation System in Humans
  Anthony K. C. Chan and Nethnapha Paredes
• 2. Platelet Physiology
  Matthew D. Linden
• 3. The Role of the Blood Vessel Wall
  Nethnapha Paredes and Anthony K. C. Chan

Part II. General Principles of Haemostatic Testing

• 4. Specimen Requirements for the Haemostasis Laboratory
  Linda Stang and Lesley G. Mitchell
• 5. Principles of Haemostasis Tests
  Gemma Crighton
• 6. Reference Ranges of Coagulation Tests
  Oliver Speer, Markus Schmugge, Claudia Metzger, and Manuela Albisetti
• 7. Lupus Anticoagulant Testing
  Karen Moffat, Anne Raby, and Mark Crowther

Part III. Examples of Global Tests of Haemostasis

• 8. Activated Partial Thromboplastin Time (APTT)
  Vera Ignjatovic
• 9. Prothrombin Time (PT) and International Normalised Ratio (INR)
  Vera Ignjatovic
• 10. Thrombin Clotting Time (TCT)
  Vera Ignjatovic
• 11. Thrombin Generation
  Leslie R. Berry and Anthony K. C. Chan
• 12. Activated Clotting Time (ACT)
  Stephen Horton and Simon Augustin

Part IV. Examples of Tests for Bleeding Disorders

• 13. Factor XII Assays
  Stephen Opat, Jenny Butler, Erica Malan, Elizabeth Duncan, and Huyen A.M. Tran
• 14. Fibrinogen
  Linda Stang and Lesley G. Mitchell
• 15. Platelet Counting
  Sylvain Salignac, Véronique Latger-Cannard, Nicole Schlegel, and Thomas Lecompte
• 16. Platelet Morphology Analysis
  Véronique Latger-Cannard, Odile Fenneteau, Sylvain Salignac, Thomas Lecompte, and Nicole Schlegel
• 17. Light Transmission Aggregometry
  Juan Pablo Frontroth
• 18. Platelet Flow Cytometry
  Matthew D. Linden

Part V. Examples of Specific Assays Related to Anticoagulant Activity

• 19. Anti-factor Xa assay (anti-Xa)
  Fiona Newall
• 20. Reptilase Time (RT)
  Hratsch Karapetian
• 21. Protamine Titration
  Fiona Newall
• 22. Laboratory Methods for the Assay if Tissue Factor Pathway Inhibitor (TFPI) in Human Plasma
  Robyn Summerhayes
• 23. Heparin Induced Thrombocytopenia (HIT)
  Andreas Greinacher, Birgitt Fürll, and Sixten Selleng

Part VI. Examples of Tests for Inhibitors of Coagulation

• 24. Nijmegen-Bethesda Assay to Measure Factor VIII Inhibitors
  Elizabeth Duncan, Margaret Collecutt, and Alison Street
• 25. Kaolin Clotting Time
  Kottayam Radhakrishnan
• 26. The Dilute Russell Viper Venom Time (DRVVT)
  Kottayam Radhakrishnan
• 27. Platelet Neutralisation Test
  Kottayam Radhakrishnan

Part VII. Examples of Tests for Increased Risk of Thrombosis

• 28. Antithrombin
  Mirta Hepner and Vasiliko Karlaftis
• 29. Protein C
  Mirta Hepner and Vasiliki Karlaftis
• 30. Protein S
  Mirta Hepner and Vasiliki Karlaftis
• 31. Testing for Hyperhomocysteinemia in Subjects with a History of Thromboembolic Events Using HPLC Technique
  Jonas Denecke
• 32. Anticardiolipin Antibody and Anti-Beta 2 Glycoprotetin I Assays
  Anne Raby, Karen Moffat, and Mark Crowther
• 33. Testing for Apolipoprotein (a) Phenotype Using Isoelectric Focusing and Immunoblotting Technique
  Claus Langer, Bertram Tambyrayah, and Ulrike Nowak-Göttl

Part VIII. Examples of Measures of Fibrinolysis

• 34. D-Dimer and Fibrinogen/Fibrin Degradation Products
  Linda Stang