Evolving technologies starting with tracer studies, and more recently with genomics and proteomics, have provided novel information about the molecular properties of cerebral endothelium and astrocytes; however, further studies must be done in animal models of neurological diseases and in humans to get a clearer understanding of the pathogenesis of blood-brain barrier (BBB) breakdown in nervous system diseases. In The Blood-Brain and Other Neural Barriers: Reviews and Protocols, experts in the field present a series of cutting-edge protocols which can be used to study the barriers. Opening with detailed information on components of the neurovascular unit as well as the blood-cerebrospinal, blood-retinal, and blood-nerve barriers, the book continues with meticulous techniques to image the barriers in humans and experimental animals, followed by cutting-edge molecular techniques to study the BBB and novel models to study the barriers, and it concludes with techniques for the delivery of therapeutic agents across the BBB. Written in the highly successful Methods in Molecular Biology™ series format, protocol chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, The Blood-Brain and Other Neural Barriers: Reviews and Protocols will aid the research efforts of not only graduate students but also more experienced investigators and support future studies of these vital systems.
Content Level » Professional/practitioner
Keywords » BBB breakdown - Blood-brain barrier - Blood-cerebrospinal barrier - Blood-nerve barrier - Blood-retinal barrier - Brain endothelial cells - Cerebral endothelium - Pericytes, astrocytes, & neurons - Therapeutic agents
Related subjects » Human Physiology - Neuroscience
TABLE OF CONTENTS
Part I: Biology of the Barriers 1. Morphology and Properties of Brain Endothelial Cells Sukriti Nag 2. Morphology and Properties of Pericytes Paula Dore-Duffy and Kristen Cleary 3. Morphology and Properties of Astrocytes Sukriti Nag 4. The Blood-Cerebrospinal Fluid Barrier: Structure and Functional Significance Conrad E. Johanson, Edward G. Stopa, and Paul N. McMillan 5. The Blood-Retinal Barrier: Structure and Functional Significance E. Aaron Runkle and David A. Antonetti 6. The Blood-Nerve Barrier: Structure and Functional Significance Ananda Weerasuriya and Andrew P. Mizisin Part II: Imaging the Barriers 7. Detection of Multiple Proteins in Intracerebral Vessels by Confocal Microscopy Janet L. Manias, Anish Kapadia, and Sukriti Nag 8. Multiparametric Magnetic Resonance Imaging and Repeated Measurements of Blood-Brain Barrier Permeability to Contrast Agents Tavarekere N. Nagaraja, Robert A. Knight, James R. Ewing, Kishor Karki, Vijaya Nagesh, and Joseph D. Fenstermacher 9. Detection of Brain Pathology by Magnetic Resonance Imaging of Iron Oxide Microparticles Daniel C. Anthony, Nicola R. Sibson, Martina A. McAteer, Ben Davis, and Robin P. Choudhury 10. Measuring the Integrity of the Human Blood-Brain Barrier Using Magnetic Resonance Imaging Andrea Kassner and Rebecca Thornhill 11. Assessing Blood-Cerebrospinal Fluid Barrier Permeability in the Rat Embryo Norman R. Saunders, C. Joakim Ek, Mark D. Habgood, Pia Johansson, Shane Liddelow, and Katarzyna M. Dziegielewska 12. Detection of Blood-Nerve Barrier Permeability by Magnetic Resonance Imaging Carsten Wessig Part III: Molecular Techniques to Study the Blood-Brain Barrier 13. Isolation of Human Brain Endothelial Cells and Characterization of Lipid Raft-Associated Proteins by Mass Spectroscopy Romain Cayrol, Arsalan S. Haqqani, Igal Ifergan, Aurore Dodelet-Devillers, and Alexandre Prat 14. Analysis of Mouse Brain Microvascular Endothelium Using Laser Capture Microdissection Coupled with Proteomics Nivetha Murugesan, Jennifer A. Macdonald, Qiaozhan Lu, Shiaw-Lin Wu, William S. Hancock, and Joel S. Pachter 15. Molecular and Functional Characterization of P-Glycoprotein In vitro Gary N.Y. Chan and Reina Bendayan 16. Methods to Study Glycoproteins at the Blood-Brain Barrier Using Mass Spectrometry Arsalan S. Haqqani, Jennifer J. Hill, James Mullen, and Danica B. Stanimirovic Part IV: Models to Study the Barriers 17. Novel Models for Studying the Blood-Brain and Blood-Eye Barriers in Drosophila Robert L. Pinsonneault, Nasima Mayer, Fahima Mayer, Nebiyu Tegegn, and Roland J. Bainton 18. Zebrafish Model of the Blood-Brain Barrier: Morphological and Permeability Studies Brian P. Eliceiri, Ana Maria Gonzalez, and Andrew Baird 19. Methods to Assess Pericyte-Endothelial Cell Interactions in a Co-Culture Model Gokulan Thanabalasundaram, Jehad El-Gindi, Mira Lischper, and Hans-Joachim Galla 20. Isolation and Properties of an In vitro Human Outer Blood-Retinal Barrier Model Robin D. Hamilton and Lopa Leach 21. Isolation and Properties of Endothelial Cells Forming the Blood-Nerve Barrier Yasuteru Sano and Takashi Kanda Part V: Delivery of Therapeutic Agents Across the Barriers 22. Treatment of Focal Brain Ischemia with Viral Vector-Mediated Gene Transfer Hua Su and Guo-Yuan Yang 23. Blood-Brain Barrier Disruption in the Treatment of Brain Tumors Marie Blanchette and David Fortin 24. Integrated Platform for Brain Imaging and Drug Delivery Across the Blood-Brain Barrier Umar Iqbal, Abedelnasser Abulrob, and Danica B. Stanimirovic 25. Targeting the Choroid Plexus-CSF-Brain Nexus Using Peptides Identified by Phage Display Andrew Baird, Brian P. Eliceiri, Conrad E. Johanson, Edward G. Stopa, Wendy Leadbeater, and Ana Maria Gonzalez