Diagnostic Virology Protocols (Methods in Molecular Biology, Vol. 665)

About this book

Recent outbreaks of swine influenza and avian influenza, along with the remaining and in some cases expanding threats from HIV, dengue virus, and the viruses causing hepatitis, have reinforced the need for rapid, accurate and cost-effective diagnosis of viral disease. Diagnostic Virology Protocols, Second Edition brings the field fully up-to-date with a focus on protocols involving nucleic acid detection, most often through some form of the polymerase chain reaction (PCR). The expert contributors also delve into the key technology of robotics as well as future prospects, such as further refined point-of-care testing and the increasing importance of mathematical modelling. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Diagnostic Virology Protocols, Second Edition captures the dramatic changes in the virus diagnostic laboratory in order to better prepare scientists to combat the inevitable threats to public health from future and present infectious diseases.

Content Level » Professional/practitioner
Keywords » Mathematical modelling - Microfluidics systems - Nucleic acid detection - Polymerase chain reaction (PCR) - Robotic analyzers - Viruses
Related subjects » Immunology - Virology

Table of contents

1. The Detection of Adenoviruses Kanti Pabbaraju, Sallene Wong, and Julie D. Fox 2. Alphaviruses A.M. Powers and John T. Roehrig 3. Detection of Human Caliciviruses in Fecal Samples by RT-PCR Robert L. Atmar, Frederick H Neill, and Françoise S. Le Guyader 4. The Detection of Dengue Virus Nagesh K. Tripathi, Ambuj Shrivastava, Paban K. Dash, and Asha M. Jana 5. Detection of Enteroviruses from Clinical Specimens Chit Laa Poh and Eng Lee Tan 6. The Detection of Hepatitis Viruses Peter Karayiannis 7. Molecular Detection of Herpesviruses Harald H. Kessler and Sophie Heyszl 8. HIV Drug Resistance Testing Patricia Cane 9. Detection, Quantification, and Characterization of HIV/SIV Neil Berry, Carolina Herrera, and Martin Cranage 10. Simultaneous Molecular Detection and Confirmation of Influenza AH5, with Internal Control Joanna S. Ellis and Martin D. Curran 11. Detection of Measles, Mumps, and Rubella Viruses Graham Tipples and Joanne Hiebert 12. Detection of High-Risk Mucosal Human Papillomavirus DNA in Human Specimens by a Novel and Sensitive Multiplex PCR Method Combined with DNA Microarray Tarik Gheit and Massimo Tommasino 13. The Detection of Parvoviruses Sean Doyle 14. The Detection and Characterization of Polioviruses Javier Martin 15. Detection of Human-Pathogenic Poxviruses Andreas Kurth and Andreas Nitsche 16. Lyssaviruses: Special Emphasis on Rabies Virus and Other Members of the Lyssavirus Genus Graeme Harkess and Anthony R. Fooks 17. Simultaneous Detection and Differentiation of Respiratory Syncytial Virus and Other Respiratory Viral Pathogens Susan E. Sefers, Haijing Li, and Yi-Wei Tang 18. Rotaviruses Jim Gray and Miren Iturriza-Gómara 19. Detection of Crimean-Congo Hemorrhagic Fever, Hanta, and Sandfly Fever Viruses by Real-Time RT-PCR Sofi M. Ibrahim, Mohamed Aitichou, Justin Hardick, Jamie Blow, Monica L. O’Guinn, and Connie Schmaljohn 20. Detection of SARS Coronavirus J. S. Malik Peiris and Leo L.M. Poon 21. The Detection of West Nile Virus Elizabeth B. Kauffman, Mary A. Franke, S.A. Wong, and Laura D. Kramer 22. Point of Care Testing: Diagnosis Outside the Virology Laboratory Christopher C. Blyth, Robert Booy, and Dominic E. Dwyer 23. Modelling Emerging Viral Epidemics for Public Health Protection Steve Leach and Ian Hall