Analgesia. Methods and Protocols

About this book

Chronic pain is a complex phenomenon, which continues to remain undertreated in the majority of affected patients thus representing a significant unmet medical need, but the development of cellular, subcellular, and molecular methods of approaching this epidemic of pain shows great promise. In Analgesia: Methods and Protocols, experts in the field present thorough coverage of molecular analgesia research methods from target discovery through target validation and clinical testing to tolerance and dependence, with extensive chapters on emerging receptor classes as targets for analgesic drugs and innovative analgesic strategies. As a volume in the highly successful Methods in Molecular Biology™ series, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes sections with tips on troubleshooting and avoiding known pitfalls.

Comprehensive and essential, Analgesia: Methods and Protocols promises to aid and enrich the research of all those scientists and clinicians who are interested in what the increasingly molecular future has in store for analgesia research, from the molecular research bench through the animal laboratory to the bedside.

Written for: Professional/practitioner

Keywords:

Analgesic drugs
Chronic pain
Personalized medicine
Single nucleotide polymorphisms (SNPs)
Target discovery and validation

Table of contents

• 1. Alternatives to Mammalian Pain Models 1: Use of C. elegans for the Study of Volatile Anesthetics
  Louise M. Steele, Margaret M. Sedensky, and Phil G. Morgan
• 2. Alternatives to Mammalian Pain Models 2: Using Drosophila to Identify Novel Genes Involved in Nociception
  Jason C. Caldwell and W. Daniel Tracey, Jr.
• 3. Animal Models of Acute Surgical Pain
  Hyangin Kim, Backil Sung, and Jianren Mao
• 4. Animal Models of Acute and Chronic Inflammatory and Nociceptive Pain
  Janel M. Boyce-Rustay, Prisca Honore, and Michael F. Jarvis
• 5. Noxious Heat Threshold Measured with Slowly Increasing Temperatures: Novel Rat Thermal Hyperalgesia Models
  Kata Bölcskei, Gábor Petho, and János Szolcsányi
• 6. Locomotor Activity in a Novel Environment as a Test of Inflammatory Pain in Rats
  David J. Matson, Daniel C. Broom, and Daniel N. Cortright
• 7. Rationale and Methods for Assessment of Pain-Depressed Behavior in Preclinical Assays of Pain and Analgesia
  S. Stevens Negus, Edward J. Bilsky, Gail Pereira Do Carmo, and Glenn W. Stevenson
• 8. Animal Models of Orofacial Pain
  Asma Khan and Kenneth M. Hargreaves
• 9. Migraine Models
  Silvia Benemei, Francesco De Cesaris, Paola Nicoletti, Serena Materazzi, Romina Nassini, and Pierangelo Geppetti
• 10. Experimental Models of Visceral Pain
  Mia Karpitschka and Martin E. Kreis
• 11. Human Correlates of Animal Models of Chronic Pain
  Arpad Szallasi
• 12. Human Experimental Pain Models 1: The Ultraviolet Light UV-B Pain Model
  James Modir and Mark Wallace
• 13. Human Experimental Pain Models 2: The Cold Pressor Model
  James Modir and Mark Wallace
• 14. Human Experimental Pain Models 3: Heat/Capsaicin Sensitization and Intradermal Capsaicin Models
  James Modir and Mark Wallace
• 15. The Value of the Dental Impaction Pain Model in Drug Development
  Stephen A. Cooper and Paul J. Desjardins
• 16. Live Cell Imaging for Studying G Protein-Coupled Receptor Activation in Single Cells
  Deepak Kumar Saini and Narasimban Gautam
• 17. Recombinant Cell Lines Stably Expressing Functional Ion Channels
  Florian Steiner, Sraboni Ghose, and Urs Thomet
• 18. Ion Channels in Analgesia Research
  Tamara Rosenbaum, Sidney A. Simon, and Leon D. Islas
• 19. Electrophysiological and Neurochemical Techniques to Investigate Sensory Neurons in Analgesia Research
  Alexandru Babes, Michael J.M. Fischer, Gordon Reid, Susanne K. Sauer, Katharina Zimmermann, and Peter W. Reeh
• 20. The Genetics of Pain and Analgesia in Laboratory Animals
  William R. Lariviere and Jeffrey S. Mogil
• 21. RT-PCR Analysis of Pain Genes: Use of Gel-Based RT-PCR for Studying Induced and Tissue-Enriched Gene Expression
  Kendall Mitchell and Michael J. Iadarola
• 22. Gene-Based Approaches in the Study of Pathological Pain
  Elisa Dominguez, Alice Meunier, and Michel Pohl
• 23. Linkage Analysis and Functional Evaluation of Inherited Clinical Pain Conditions
  Johannes J. Krupp, Dennis Hellgren, and Anders B. Eriksson
• 24. Rat Bone Marrow Stromal Cells and Oligonucleotides in Pain Research
  María Florencia Coronel, Norma Alejandra Chasseing, and Marcelo José Villar
• 25. Transplantation of Human Mesenchymal Stem Cells in the Study of Neuropathic Pain
  Dario Siniscalco
• 26. Delivery of RNA Interference to Peripheral Neurons in vivo Using Herpes Simplex Virus
  Anna-Maria Anesti
• 27. Combination of Cell Culture Assays and Knockout Mouse Analyses for the Study of Opioid Partial Agonism
  Soichiro Ide, Masabumi Minami, Ichiro Sora, and Kazutaka Ikeda
• 28. Assessing Potential Functionality of Catechol-O-methyltransferase (COMT) Polymorphisms Associated with Pain Sensitivity and Temporomandibular Joint Disorders
  Andrea G. Nackley and Luda Diatchenko
• 29. Genetic Polymorphisms and Human Sensitivity to Opioid Analgesics
  Daisuke Nishizawa, Masakazu Hayashida, Makoto Nagashima, Hisashi Koga, and Kazutaka Ikeda
• 30. Molecular Assays for Characterization of Alternatively Spliced Isoforms of the Mu Opioid Receptor (MOR)
  Pavel Gris, Philip Cheng, John Pierson, William Maixner, and Luda Diatchenko
• 31. Inhalational Anesthetic Photolabeling
  Roderic G. Eckenhoff, Jin Xi, and William P. Dailey
• 32. Measuring Membrane Protein Interactions Using Optical Biosensors
  Candice Davidoff, Benjamin J. Doranz, and Joseph Rucker
• 33. Proteomics and Metabolomics and Their Application to Analgesia Research
  Nichole A. Reisdorph and Richard Reisdorph
• 34. Preemptive Analgesia: Problems with Assessment of Clinical Significance
  Igor Kissin
• 35. Standardization of Pain Measurements in Clinical Trials
  William K. Sietsema
• 36. Procedural Sedation and Analgesia Research
  James R. Miner
• 37. Non-Invasive Transcranial Direct Current Stimulation for the Study and Treatment of Neuropathic Pain
  Helena Knotkova and Ricardo A. Cruciani
• 38. Pain Imaging in the Emerging Era of Molecular Medicine
  Christian S. Stohler and Jon-Kar Zubieta
• 39. Current and Emerging Pharmacologic Therapies for Pain and Challenges Which Still Lay Ahead
  Christopher Noto and Marco Pappaga