ABOUT THIS BOOK
Recent insight into the transcripts generated from the mammalian genome (i.e.
the transcriptome) has revealed that transcription is a far more complex phenomenon
than previously thought. In RNA: Methods and Protocols, expert researchers provide
the procedures and methods used to describe the structure of messenger RNAs
and non-coding RNAs that are transcribed by RNA polymerase II as the immediate
gene products in mammalian cells. Focused on the structure of the RNA products
of “gene X” and the mapping of proteins associated with these RNAs,
the volume presents appropriate information for non-specialists in RNA biology.
Written in the highly successful Methods in Molecular Biology™ series
format, many chapters contain introductions to their respective topics, lists
of the necessary materials and reagents, step-by-step, readily reproducible
laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Comprehensive and practical, RNA: Methods and Protocols views the transcriptional
landscape with an appreciation for the role that proteins play in the processing
and interpretation of genetic information in an attempt to further our crucial
knowledge of the many products and sophisticated regulatory networks that result
Content Level » Professional/practitioner
Keywords » Bioinformatics - Mammalian genome - Messenger RNAs - Microarray analysis - Non-coding RNAs - Protein mapping - RNA polymerase II - RNP methods - Transcriptional landscape - Transcriptome - miRNA profiling
Related subjects » Human Genetics