ABOUT THIS BOOK
We have entered the era of molecular immunohematology in which DNA is analyzed
to predict red blood cell and platelet phenotypes. Indeed, many laboratories
and blood banks across the world have already adopted and implemented molecular
techniques. Some fear that serology is now becoming outdated, but immunohematology
techniques often supplement the work of serologists, and several leading serology
laboratories are the very same ones that were early adopters of molecular immunohematology.
This book is a thorough review of molecular immunohematology and is aimed at
anyone interested in how it is changing blood bank and transfusion medicine.
BeadChip Molecular Immunohematology opens with a history of serology before
it provides an overview of gene expression, how genetic variations translate
into antigenic differences on the surfaces of cells, and techniques that have
been developed to detect genetic variations. It then describes in detail the
BeadChip technology developed by BioArray Solutions, the molecular immunohematology
system that has been most widely adopted in blood blank laboratories. The remainder
of the book focuses on analyses and case studies from around the world to describe
how molecular immunohematology is being used in various settings for various
purposes. It includes a discussion of the significant advances that have been
made to incorporate Rh testing on the BeadChip platform as well as the potential
use of that system.
Content Level » Professional/practitioner
Related subjects » Biomedical Sciences - Immunology - Internal Medicine - Molecular Medicine
TABLE OF CONTENTS
Chapter 1: An overview of the Classical Serological methods: limitations and benefits of serology and DNA testing John J. Moulds Chapter 2: Introduction to Molecular Typing Joann M. Moulds, Steven R. Sloan* Chapter 3: The BeadChip System: A Flexible Array Format for Complex Nucleic Acid and Protein Analysis Ghazala Hashmi*, Yi Zhang, Michael Seul Chapter 4: Implementation of HEA at Blood Centers: Prescreening, Rare Donors, Inventory Management Joann M. Moulds Chapter 5: Implementation of HEA BeadChip System at Medical Centers: Providing Extended Matched Units and Eliminating Complex Workups for Patients Ellen Klapper Chapter 6: Human Platelet Antigen Genotyping and Diagnosis of Antiplatelet Alloimmunization Gerald Bertrand, Cecile Kaplan-Gouet* Chapter 7: Blood group genotyping by high-throughput DNA-analysis: Application to the Panel National de Référence du CNRGS Sandrine Kappler-Gratias, Thierry Peyrard, Pierre Yves Le Pennec, Jean-Pierre Cartron, Philippe Rouger, Bach-Nga Pham* Chapter 8: Implementation and Assessment of High-Throughput Donor Typing at the Milan, Italy Immunohematology Reference Laboratory Nicoletta Revelli*, Cinzia Paccapelo, Paola Ponzo, Francesca Truglio, Veronica Sala, Francesca Poli, Maurizio Marconi, Maria Antonietta Villa Chapter 9: Implementation of the BioArray Erythrocyte Antigen (HEA) genotyping System BeadChip™ HEA: The Red Cross of Spain Experience Luisa Barea García, Marta Martinez Sánchez, Gemma Cabrera Rodriguez-Escalona, Emma Castro Izaguirre* Chapter 10: Looking Beyond HEA: Matching SCD Patients for RH Variants Marion E Reid*, Christine Halter Hipsky Chapter 11: Identification of Altered RHD and RHCE Alleles: A Comparison of Manual and Automated Molecular Methods Sunitha Vege, Connie M. Westhoff* Chapter 12: Bayesian Classification Algorithms for Automated Allele Assignment Kairali Podual, Yi Zhang, Natalia Mezokh, JiaCheng Yang, Randall Wilson*