Atlas of Comparative Diagnostic and Experimental Hematology

DESCRIPTION

A vital resource on blood and bone marrow cell morphology in laboratory animal medicine. This fully revised new edition is an essential reference for clinical pathologists in diagnostic laboratories, and medical or veterinary research. The atlas contains over 400 color images of cells from the peripheral blood and bone marrow from a variety of animals encountered in laboratory animal medicine, in health and disease.

FEATURES

• Over 400 color images of both normal and abnormal cells
• New chapter on flow cytometry and its application in terms of routine analyses as a means of identifying abnormalities in cell marker expression, which is of particular relevance for pre-clinical safety assessment
• Covers the most recent developments in laboratory animal hematology, including parameters measured by the latest generation of analyzers
• Coverage of a wide range of laboratory animal species, as well as those used in clinical veterinary trials
• Photomicrographs present normal and abnormal blood cells from a variety of hematological conditions along with descriptive text

TABLE OF CONTENTS

Foreword vii
Introduction ix
Dedication xi
Acknowledgments xiii

Rodents 1

• Mouse 1
  • Introduction 1
  • Blood picture 1
  • Typical ranges (Siemens Advia 120) 2
• Hamster 15
  • Introduction 15
  • Blood picture 15
• Guinea Pig 17
  • Introduction 17
  • Blood picture 17
• Rat – Sprague Dawley (SD)/Han Wistar (HW) 18
  • Introduction 18
  • Blood picture 18
  • Typical ranges (Siemens Advia 120) 19
  • References 36
• Rabbit 37
  • Introduction 37
  • Blood picture 37
  • References 39

Cat 41

• Introduction 41
• Blood picture 41
• References 44

Dog 45

• Introduction 45
• Blood picture 45
• Typical ranges (Siemens Advia 120) 46
• References 64

Monkey 65

• Introduction 65
• Blood picture 65
• Marmoset 66
• Cynomolgus 71
  • Introduction 71
  • Blood picture 71
  • Typical ranges (Siemens Advia 120) 72
• Baboon 84
• Rhesus Monkey 86
• References 87

Other species 89

• Pig 89
  • Introduction 89
  • Blood picture 89
• Horse 91
  • Introduction 91
  • Blood picture 91
• Goat and Sheep 94
• Assorted Other Species 96
• References 103

Blood collection procedures 105

• Common bleeding sites 105
• Anticoagulants 105
• Staining 105

Artifacts 107

• Collection artifacts 107
• Sample transport and storage 109
• Anticoagulant 110
• Storage artifacts 111
• Blood smear preparation 113
• Stain precipitate 113
• References 114

Bone marrow 115

• Introduction 115
• Review of marrow sampling techniques 115
• Recommended methods for marrow sampling 116
• Preparation and staining of smears 118
• Evaluation 119
• Interpretation 119
• References 120

Comparative applications in flow cytometry 121

• Application of fl ow cytometry in comparative hematology 122
• References 132

Index 135

AUTHOR

Clifford Smith is Chief Laboratory Scientist in the Haematology Department, and Head of Logistics, Clinical Pathology Services, at Covance Laboratories in Harrogate, UK.
Alfred Jarecki is Principal Research Scientist with Sanofi-Aventis at the Alnwick Research Centre, UK.