This practical manual provides a comprehensive yet concise guide to state-of-the-art molecular techniques and their applications. It starts with an overview of the essential principles of molecular techniques, followed by separate chapters detailing the use of these techniques in particular tissues and organs, and describing recommended treatment plans. Each chapter covers the tests available, their advantages, limitations, and use as diagnostic and prognostic tools, with key learning points at the end of each topic. Using both histologic and cytologic samples, it discusses how to interpret test results in a pathologic context and enables trainees and practising pathologists to gain an in-depth understanding of molecular diagnostic techniques and how to incorporate them into routine diagnostic practice. Aiding the daily practice of refining diagnosis, as well as offering a didactic approach, this book is an essential reference for practising pathologists and cytopathologists as well as trainees in pathology.
- Offers a system-specific molecular approach in tissues and cytological preparations and covers current and future trends in molecular pathology to familiarize pathologists and cytopathologists with molecular diagnostic and prognostic criteria
- Describes the application of molecular techniques in diagnostic histopathology, cytopathology and clinical management
- Each chapter ends with a set of key learning points, offering a summary as well as a learning tool to surgical pathologists and cytopathologists
List of contributors
1. An introduction to molecular pathology John M. S. Bartlett
2. Molecular regulation of cellular function Marsela Braunstein and John M. S. Bartlett
3. Practical application of molecular techniques in diagnostic histopathology and cytopathology and clinical management Abeer Shaaban and Fernando Schmitt
4. Fluorescent and non-fluorescent in situ hybridization Jane Bayani and John M. S. Bartlett
5. Clinical applications of the polymerase chain reaction for molecular pathology Angela B. Y. Hui, Annette D. Wong and Carlo V. Hojilla
6. Are microarrays ready for prime time? Jane Bayani and John M. S. Bartlett
7. Tissue microarrays Luigi Tornillo
8. Sequencing Andrew M. K. Brown, Philipe C. Zuzarte and Paul M. Krzyzanowski
9. Microsatellite instability in colorectal cancer Maria Sofia Fernandes, Raquel Seruca and Sérgia Velho
10. Mutational analysis Aaron R. Hansen, Mahadeo A. Sukhai, Hal K. Berman, Suzanne Kamel-Reid and Philippe L. Bedard
11. Tumors of the breast Cathy B. Moelans, Petra van der Groep, Robert Kornegoor, Jelle Wesseling and Paul J. van Diest
12. Tumors of the female genital system Adhemar Longatto Filho, Sigurd F. Lax and Bin Yang
13. Tumours of the male urogenital system Kirsten D. Mertz and Lukas Bubendorf
14. Tumors of the gastrointestinal system Manuel Salto-Tellez, Benedict Yan, Roseann I. Wu and Martha Bishop Pitman
15. Soft tissue, bone and skin tumours Stamatios Theocharis and Jerzy Klijanienko
16. Cardio-thoracic tumors Umberto Malapelle, Claudio Bellevicine, Elena Vigliar and Giancarlo Troncone
17. Tumors of the endocrine system Sylvia L. Asa and Ozgur Mete
18. Haematological malignancies of the lymph nodes Sarah E. Coupland and Pedro Farinha
19. Pediatric tumors Paul Roberts, Susan Picton and Jens Stahlschmidt
20. Serous effusions Ben Davidson
21. Academic applications and future of molecular pathology Manuel Salto-Tellez and Benedict Yan
John M. S. Bartlett, Ontario Institute for Cancer Research, Toronto
John M. S. Bartlett, PhD, FRCPath, is Director of Transformative Pathology and Improved Management of Early Cancer at the Ontario Institute for Cancer Research, Toronto, Ontario, Canada.
Abeer Shaaban, University Hospitals Birmingham NHS Foundation Trust
Abeer Shaaban is Consultant Pathologist at the Department of Cellular Pathology, Queen Elizabeth Hospital, Birmingham, UK, and Lecturer at the Department of Pathology, Tanta University Hospital, Tanta, Egypt.
Fernando Schmitt, Laboratoire National de Santé, Luxembourg
Fernando Schmitt is Director of the Department of Medicine and Pathology at the Laboratoire National de Sante, Dudelange, Luxembourg.