Dna Microarrys. a Molecular Cloning Manual

Description

DNA microarray technology is a new and powerful means to analyze genomes and characterize patterns of gene expression. Its applications are widespread across the many fields of plant and animal biological and biomedical research. This manual, designed to extend and to complement the information in the best–selling Molecular Cloning, is a synthesis of the expertise and experience of more than 30 contributors—all innovators in a fast–moving field. DNA Microarrays provides authoritative, detailed instruction on the design, construction, and applications of microarrays, as well as comprehensive descriptions of the software tools and strategies required for analysis of images and data.

Contents

• SECTION 1: GENERATION OF PROBES FOR SPOTTED MICROARRAYS
  S. Chandrasekharappa, A. Holloway, V. Iyer, D. Monte, M. Murphy, N.J. Nowak
• SECTION 2: PRINTING SPOTTED GLASS MICROARRAYS
  G. Childs, J. DeRisi, T. Harris, A. Holloway, B.–H. Hou, A. Massimi, M. Murphy, S. Somerville
• SECTION 3: EXPRESSION ANALYSIS OF RNA
  M. Bittner, R. Butow, J. DeRisi, M. Diehn, J. Eberwine, C. Epstein, R. Glynne, S. Grimmond, T. Ideker, J.E. Kacharmina, S. Katsabanis, J. Khan, J. Lee, C.L. Liu, P. Marciano, F.M. Marincola, T. McIntosh, D. Monte, J.R. Pollack, V. Rhodius, S. Somerville, E. Tom, E. Wang, J.S. Wei, D. Willhite, S. Ybarra
• SECTION 4: MEMBRANE–BASED SPOTTED cDNA ARRAYS
  K. Becker, W.H. Wood III, C. Cheadle
• SECTION 5: TISSUE MICRODISSECTION
  B. Becker, M. Bernsen, C.J.M. Best, T. Björnsen, D. Brocksch, R. Burgemeister, C. Bush, R.F. Chuaqui, A. Clement–Sengewald, M.R. Emmert–Buck, G. Friedemann, J.W. Gillespie, W. Heckl, G. Lahr, I.M. Leiva, P. Lindahl, A. Mayer, S. Nilsson, S.J. Scheidl, K. Schütze, M. Stich, W. Stolz, M. Takemoto, S. Thalhammer, M.C.R.F. van Dijk, T. Vogt
• SECTION 6: USING MICROARRAYS FOR GENOMIC ANALYSIS
  D. Albertson, S. Dee, J.–B. Fan, J.N. Hirschhorn, X. Huang, V. Iyer, Y.–M. Lim, K. Lindblad?Toh, R. Lucito, H. Matsuzaki, N. Patil, D. Pinkel, J.R. Pollack, T. Ryder, R. Segraves, P. Sklar, A. Snijders, M. Wigler
• SECTION 7: AN INTRODUCTION TO MICROARRAY BIOINFORMATICS
  C.A. Ball, Y. Chen, S. Panavally, G. Sherlock, T. Speed, P.T. Spellman, Y.H. Yang
• SECTION 8: TISSUE MICROARRAYS
  K. Brand, G. Hostetter, O.–P. Kallioniemi, J. Kononen, G. Sauter, M. Trivett
• APPENDIX 1: Generation of Full-length Libraries
  P. Carninci
• APPENDIX 2: Reagents, Buffers, and Media
• APPENDIX 3: Cautions
• APPENDIX 4: Suppliers