Laboratory Investigations in Molecular Biology presents well-tested protocols in molecular biology that are commonly used in currently active research labs. It is an ideal laboratory manual for college level courses in molecular biology. Because of the modular organization of the manual, laboratory courses can be assembled that would be ideal for science professionals, graduate students, undergraduate students and even advanced high school students in AP courses. The manual is also intended to be useful as a laboratory "bench reference". The experiments are designed to guide students through realistic research projects and to provide students with instruction in methods and approaches that can be immediately translated into research projects conducted in modern research laboratories. Although these experiments have been conducted and optimized over 20 years of teaching the New England Biolabs Molecular Biology Summer Workshops, they are real research projects, not "canned" experiments. Based on extensive teaching experience using these protocols, the authors have found that conducting these experiments as described in these protocols serves to effectively instruct students and science professions in the basic methods of molecular biology. An additional unique feature is that the protocols described in the manual are accompanied by available reagent kits that provide quality-tested, pre-packaged reagents to ensure the successful application of these protocols in a laboratory course setting.
Laboratory courses appropriate for this lab manual include those at the Bachelors level and would also be ideal for a laboratory course for students pursuing Masters or PhD degrees. The text is also ideal for courses in any other specialized degree that requires, basic knowledge of techniques in molecular biology (e.g. biotechnology, forensic medicine, molecular medicine). This text can also be adapted for use at the junior college level or the advanced high school level. The book also stands alone as a fine addition to any research laboratory as a reference work in Laboratory Molecular Biology
- Describes well-tested protocols for procedures in molecular biology that are commonly used in currently active research labs.
- The protocols described in the text are the product of over 20 years of thorough testing in the New England Biolabs Molecular Biology Summer Workshops.
- Forms the basis for a sophisticated laboratory course in molecular biology that prepares students for independent research work in a research laboratory.
- The text is flexible enough to fit the needs of a variety of course lengths and levels of difficulty, from junior college through graduate courses, and professional development.
- The experiments are all real research projects that are designed in a modular format for ease in adapting the projects to one or two semester laboratory courses with either one or two lab sessions per week.
- Reagents are available as a companion to each module to make the experiments easier for instructors to prepare and to dramatically improve the likelihood of success with the experiments.
- The text will also prove useful as a laboratory reference manual for any research group using molecular biology techniques.
TABLE OF CONTENTS
Project I. Genomic DNA Isolation and Analysis
Module I.1 Mouse Genomic DNA Isolation and Analysis
ABOUT THE AUTHORS
Steven A. Williams, PhD - Smith College and The University of Massachusetts, Massachusetts
Dr. Williams received his Ph.D. in Genetics from the University of California, Davis in 1982. His doctoral dissertation research focused on gene expression in the Yeast Saccharomyces cerevisiae. Dr. Williams has been at Smith College and the University of Massachusetts since 1982. In 1986, he founded (with Dr. Barton Slatko) the New England Biolabs Molecular Biology Summer Workshops. These two week workshops are the longest running and largest molecular biology courses for professionals in the world. In 1994, Dr. Williams was named the director of the Filarial Genome Project by the World Health Organization. In 1995, the Clark Foundation named Dr. Williams the director of the River Blindness Genome Project. In 2005, he was named Director of the new Center for Molecular Biosciences at Smith College. Dr. Williams research accomplishments and interests include the discovery and characterization of the first repetitive DNA sequences in several species of parasitic nematodes, the development of sensitive PCR diagnostic tests for these parasites and the sequencing of over 5000 filarial parasite genes. His laboratory research focuses on the application of genomics, bioinformatics, RNAi, and microarrays to the study of gene expression in the parasites that cause Lymphatic Filariasis, Elephantiasis, and African River Blindness.
Barton E. Slatko, PhD - New England Biolabs, Inc.
Dr. Slatko received his Ph.D. degree in Zoology Genetics from the University of Texas, Austin in 1977. His doctoral dissertation research was conducted in the laboratory of Dr. Yuichiro Hiraizumi and was focused on the genetics and cytogenetics of the male recombination/mutator strains of Drosphila melanogaster, now known to be due to transposable P elements and their associated cytotypes. He then did a postdoctoral fellowship in the laboratory of Dr. Mel Green at The University of California, Davis. Dr. Slatko was as Assistant Professor at Williams College (Williamstown, MA) before joining New England Biolabs, Inc. (NEB) in 1983. Dr. Slatko was part of the restriction endonuclease group and molecularly cloned the first thermophilic restriction endonuclease and cognate methylase (TaqI), before forming the DNA sequencing core facility at NEB. Since that time, he has been part of the Parasitology Division involved in the Filarial Genome Project, which involves the goal of eradication filarial diseases from worldwide human populations. Under his direction, his laboratory recently completed and published the DNA sequence and annotation of an obligate endosymbiont (Wolbachia) from a filarial parasite, a potential therapeutic and preventative drug target. He has served on both NSF and NIH grant review panels and is a science editor for BioTechniques. While maintaining his research laboratory, he now heads the new product acquisition and development group for NEB. He is actively involved in science education and has been involved with the NEB Molecular Biology Courses, with the other co-authors, since 1986.
John R. McCarrey, PhD - University of Texas at San Antonio, San Antionio, Texas
Dr. McCarrey received his Ph.D. degree in Genetics from the University of California, Davis in 1981. His doctoral dissertation research was conducted in the laboratory of Dr. Ursula Abbott and was focused on sex determination and germ cell development in the chick embryo. He then did a postdoctoral fellowship in the laboratory of Dr. Susumu Ohno at the City of Hope Research Institute in Duarte, California, where he worked on the molecular biology of the mammalian sex determination process. Dr. McCarrey's first faculty position was in the Division of Reproductive Biology in the Johns Hopkins University School of Hygiene and Public Health. He then moved to the Southwest Foundation for Biomedical Research in San Antonio, Texas. In 2001, he assumed his current position as Professor of Cell and Molecular Biology at the University of Texas at San Antonio. Dr. McCarrey's research accomplishments and interests include the discovery of the first functional retroposon in the human genome, elucidation of mechanisms that regulate tissue-specific transcription in mammalian spermatogenic cells, studying the spermatogenesis in mammals, describing the kinetics of reprogramming of epigenetic mechanisms during mammalian gametogenesis, and current studies to investigate the status of epigenetic and genetic mechanisms in cloned mice and in mouse embryos produced by assisted reproductive technologies, as well as in embryonic stem cells derived from mice and nonhuman primates.