This book contains all the necessary information and advice for anyone wishing to obtain electron micrographs showing the most accurate ultrastructural detail in thin sections of any type of biologicalspecimen.
The guidelines for the choice of preparative methods are based on an extensive survey of current laboratory practice. For the first time, in a textbook of this kind, the molecular events occurring duringfixation and embedding are analysed in detail. The reasons for choosing particular specimen preparation methods are explained and guidance is given on how to modify established techniques to suitindividual requirements.
All the practical methods advocated are clearly described, with accompanying tables and the results obtainable are illustrated with many electron micrographs.
Portland Press Series: Practical Methods in Electron Microscopy, Volume 17, Audrey M. Glauert, Editor
Originally published in 1999.
Table of contents
- An introduction to fixation and embedding procedures and their safe use in the laboratory
- Fixation methods
- Dehydration methods
- Embedding methods
- Embedding in epoxy resins
- Embedding in acrylic resins
- The Lowicryl resins and embedding at low temperatures
- Other embedding media
- Processing schedules
Appendix: List of suppliers