This practical, extensively illustrated handbook covers the procedures that are undertaken in andrology and ART laboratories to analyse and assess male-factor infertility, and to prepare spermatozoa for use in assisted conception therapy. The content is presented as brief, authoritative overviews of the relevant biological background for each area, plus detailed, step-by-step descriptions of the relevant analytical procedures. Each technical section includes pertinent quality control considerations, as well as the optimum presentation of results. In addition to the comprehensive ‘basic’ semen analysis, incorporating careful analysis of sperm morphology, the handbook provides established techniques for the use of computer-aided sperm analysis and sperm functional assessment. Throughout the handbook the interpretation of laboratory results in the clinical context is highlighted, and safe laboratory practice is emphasized. It is an invaluable resource to all scientists and technicians who perform diagnostic testing for male-factor infertility.
• Includes structured step-by-step instructions for basic analysis to give the reader an overview of the methods and proper implementation of techniques • Detailed instructions for sperm morphology staining with each critical step explained to help troubleshooting errors • 8 colour plates on morphology to enhance understanding
List of abbreviations; Preface; 1. Introduction; 2. Basic physiology; 3. Basic semen analysis; 4. External semen analysis; 5. Sperm function tests; 6. Sperm-mucus interaction; 7. Sperm preparation; 8. Sperm cryobanking; 9. SSR; 10. Quality management; 11. Risk management; 12. ReproTox testing; Appendices: 1. Reference values; 2. Basic andrology lab equipment list; 3. Company references; 4. ESHRE CASA consensus; 5. Home testing overview; 6. Sample forms; 7. Comparison of replicate conts; 8. Safety; Index.