Genomics research has made significant advances in recent years. In this book,
a team of internationally-renowned researchers share the most up-to-date information
in a field that has in recent years switched emphasis from gene identification
to functional genomics and the characterization of genes and gene products.
This volume approaches this complex subject with a broad perspective to supply
the reader with a vital overview of genomics and its derivative fields, with
a focus on pivotal issues such as data analysis. Expansive and current, this
book is a comprehensive research guide that describes both the key new techniques
and more established methods. Every chapter discusses the merits and limitations
of the various approaches and then provides selected tried-and-tested protocols,
as well as a plethora of good practical advice for immediate use at the bench.
- Provides a broad introduction to current practices and techniques for lab-based research in genomics
- Explains clearly and precisely how to carry out selected techniques in addition to background information on the various approaches
- Chapters are written by a leading international authorities in the field and cover both well-known and new, tried and tested, methods for working in genomics
- Includes troubleshooting guide and reviews of alternative techniques
- An essential laboratory manual for students and researchers at all levels
Table of Contents
1 High-Resolution Analysis of Genomic Copy Number Changes (Mario Hermsen, Jordy Coffa, Bauke Ylstra, Gerrit Meijer, Hans Morreau, Ronald van Eijk, Jan Oosting and Tom van Wezel).
- 1.1 Introduction.
- 1.2 Methods and Approaches.
- 1.3 Troubleshooting.
2 Identification of Polymorphic Markers for Genetic Mapping (Daniel C. Koboldt and Raymond D. Miller).
- 2.1 Introduction.
- 2.2 Methods and Approaches.
- 2.3 Troubleshooting.
3 Genotyping and LOH Analysis on Archival Tissue Using SNP Arrays (Ronald van Eijk, Anneke Middeldorp, Esther H. Lips, Marjo van Puijenbroek, Hans Morreau, Jan Oosting and Tom van Wezel).
- 3.1 Introduction.
- 3.2 Methods and Approaches.
- 3.3 Troubleshooting.
4 Genetic Mapping of Complex Traits (Nancy L. Saccone).
- 4.1 Introduction.
- 4.2 Methods and Approaches.
- 4.3 Troubleshooting.
- 4.3.1 Combining Datasets.
5 RNA Amplification Strategies: Toward Single-Cell Sensitivity (Natalie Stickle, Norman N. Iscove, Carl Virtanen, Mary Barbara, Carolyn Modi, Ari Baratz, Toni Di Berardino, Ellen Greenblatt, Ted Brown and Neil Winegarden).
- 5.1 Introduction.
- 5.2 Methods and Approaches.
- 5.3 Troubleshooting.
6 Real-Time Quantitative RT-PCR for mRNA Profiling (Stephen A. Bustin and Tania Nolan).
- 6.1 Introduction.
- 6.2 Methods and Approaches.
- 6.3 Troubleshooting.
7 Gene Expression in Mammalian Cells (Félix Recillas-Targa, Georgina Guerrero, Martín Escamilla-del-Arenal and Héctor Rincón-Arano).
- 7.1 Introduction.
- 7.2 Methods and Approaches.
- 7.3 Troubleshooting.
8 Using Yeast Two-Hybrid Methods to Investigate Large Numbers of Binary Protein Interactions (Panagoula Charalabous, Jonathan Woodsmith and Christopher M. Sanderson).
- 8.1 Introduction.
- 8.2 Methods and Approaches.
- 8.3 Troubleshooting.
9 Prediction of Protein Function (Hon Nian Chua).
- 9.1 Introduction.
- 9.2 Methods and Approaches.
- 9.3 Troubleshooting.
10 Elucidating Gene Function through Use of Genetically Engineered Mice (Mary P. Heyer, Cátia Feliciano, João Peca and Guoping Feng).
- 10.1 Introduction.
- 10.2 Methods and Approaches.
- 10.3 Troubleshooting.
11 Delivery Systems for Gene Transfer (Charlotte Lawson and Louise Collins).
- 11.1 Introduction.
- 11.2 Methods and Approaches.
- 11.3 Troubleshooting.
12 Gene Therapy Strategies: Constructing an AAV Trojan Horse (M. Ian Phillips, Edilamar M. de Oliveira, Leping Shen, Yao Liang Tang, and Keping Qian).
- 12.1 Introduction.
- 12.2 Methods and Approaches.
- 12.3 Troubleshooting.
13 An Introduction to Proteomics Technologies for the Genomics Scientist (David B. Friedman).
- 13.1 Introduction.
- 13.2 Methods and Approaches.
- 13.3 Troubleshooting.