Introduction to Proteomics gives a concise and coherent overview of every aspect of current proteomics technology. The book provides students with sufficient theoretical background to be able to plan, prepare, and analyze a proteomics study. Covering an Introduction, Separation Technologies, Analysis of Peptides/Proteins by Mass Spectrometry, and Strategies in Proteomics, this book also includes numerous examples and explanations for why particular strategies are better than others for certain applications. In addition, the book includes extensive references and a list of relevant proteomics information sources.
Table of Contents
- 1.1 What are the tasks in proteomics?
- 1.2 Challenges in Proteomics.
- 1.3 Proteomics in relation to other -omics and System Biology.
- 1.4 Some general applications of Proteomics.
- 1.5 Structure of the book.
2 Separation and Detection Technologies.
- 2.1 Introduction to experimental strategies in proteomics.
- 2.2 Gel based separation.
- 2.3 Visualization and analysis of proteins/ peptides in gels.
- 2.4 Gel Free Separation Technologies.
- 2.5. Visualization of proteins/Peptides from Hyphenated methods.
- 2.6 Chips in proteomics applications.
3 Analysis of peptides/ proteins by Mass Spectrometry.
- 3.1 Basic principles of Mass Spectrometry for proteomics.
- 3.2 Ionization Methods for small amounts of biomolecules.
- 3.3 Mass analyzers and Mass spectrometers.
- 3.4 Concluding remarks on mass analyzers for proteomics.
4 Analysis/interpretation of mass spectrometric and Proteomic Data.
- 4.1 Analysis of MS data.
- 4.2 Analysis of MS/MS data.
- 4.3 Quantification of LC MS and MS/MS data from complex samples.
- 4.4 Bioinformatic approaches for mass spectrometric proteome data analysis.
5 Strategies in Proteomics.
- 5.1 Imaging Mass spectrometry.
- 5.2 Qualitative Proteomics.
- 5.3 Differential and quantitative proteomics.
- 5.4 Analysis of posttranslational modifications.
- 5.5 Interaction-Proteomics.
- 5.6 Proteomics as part of integrated approaches.